de Kretser T A, Bodmer J G, Bodmer W F
Tissue Antigens. 1980 Oct;16(4):317-25. doi: 10.1111/j.1399-0039.1980.tb00313.x.
A technique is described for the positive separation of cell populations on the basis of antigenicity. Specific antibody is conjugated to Sepharose-4B and the resulting antibody-coated beads complexed with cells carrying the antigen against which the antibody is directed. These complexes are separated from non-complexed cells by centrifugation over Percoll. Specifically, HLA-DR positive human peripheral blood lymphocytes have been obtained with high viability and purity by the use of a monoclonal antibody directed against a determinant common to all HLA-DR antigens. This has greatly facilitated HLA-DR typing of these cells.
本文描述了一种基于抗原性对细胞群体进行阳性分离的技术。将特异性抗体与琼脂糖珠4B偶联，所得的抗体包被珠与携带该抗体所针对抗原的细胞形成复合物。通过在 Percoll 上离心，将这些复合物与未复合的细胞分离。具体而言，通过使用针对所有 HLA-DR 抗原共有的一个决定簇的单克隆抗体，已获得了具有高活力和纯度的 HLA-DR 阳性人外周血淋巴细胞。这极大地促进了这些细胞的 HLA-DR 分型。
