通过抗体偶联磁性微球进行细胞分离及其与单克隆 HLA 抗体联合应用。
Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies.
作者信息
Kandzia J, Anderson M J, Müller-Ruchholtz W
出版信息
J Cancer Res Clin Oncol. 1981;101(1):165-70. doi: 10.1007/BF00405076.
Abstract
The modification and efficiency of a technique for cell separation is described. This technique combines the specificity of serologic reactions with the strength and physical selectivity of a magnetic field. A monoclonal HLA-BW 6 antibody was coupled to 100 nm diameter Fe3O4 containing albumin microspheres via surface-incorporated St. aureus Protein-A. The mixture of HLA-BW 6 and -BW 4 human peripheral blood lymphocytes was incubated with these immunomicropheres and applied to a glass column located in a magnetic field. Only HLA-BW 4 lymphocytes passed through the column and were collected. The recovered cells were 97% viable.
摘要
描述了一种细胞分离技术的改进及其效率。该技术将血清学反应的特异性与磁场的强度和物理选择性相结合。通过表面结合的金黄色葡萄球菌蛋白A，将单克隆HLA - BW 6抗体与含直径100nm四氧化三铁的白蛋白微球偶联。将HLA - BW 6和 - BW 4人外周血淋巴细胞的混合物与这些免疫微球一起孵育，并应用于置于磁场中的玻璃柱。只有HLA - BW 4淋巴细胞通过柱子并被收集。回收的细胞存活率为97%。
相似文献
1
Cell separation by antibody-coupled magnetic microspheres and their application in conjunction with monoclonal HLA-antibodies.通过抗体偶联磁性微球进行细胞分离及其与单克隆 HLA 抗体联合应用。
J Cancer Res Clin Oncol. 1981;101(1):165-70. doi: 10.1007/BF00405076.
2
Immunosuppressive T-cell antibody induction for heart transplant recipients.心脏移植受者的免疫抑制性T细胞抗体诱导治疗
Cochrane Database Syst Rev. 2013 Dec 2;2013(12):CD008842. doi: 10.1002/14651858.CD008842.pub2.
3
First-line allogeneic hematopoietic stem cell transplantation of HLA-matched sibling donors compared with first-line ciclosporin and/or antithymocyte or antilymphocyte globulin for acquired severe aplastic anemia.与一线使用环孢素和/或抗胸腺细胞或抗淋巴细胞球蛋白治疗获得性重型再生障碍性贫血相比，HLA匹配的同胞供者进行一线异基因造血干细胞移植的情况。
Cochrane Database Syst Rev. 2013 Jul 23;2013(7):CD006407. doi: 10.1002/14651858.CD006407.pub2.
4
[Fast determination of per- and polyfluoroalkyl substances in human serum by cold-induced phase separation coupled with liquid chromatography-tandem mass spectrometry].[冷诱导相分离结合液相色谱-串联质谱法快速测定人血清中的全氟和多氟烷基物质]
Se Pu. 2025 Jul;43(7):756-766. doi: 10.3724/SP.J.1123.2024.11028.
5
Polyclonal and monoclonal antibodies for treating acute rejection episodes in kidney transplant recipients.用于治疗肾移植受者急性排斥反应的多克隆抗体和单克隆抗体。
Cochrane Database Syst Rev. 2017 Jul 20;7(7):CD004756. doi: 10.1002/14651858.CD004756.pub4.
6
Preventive drug treatments for adults with chronic migraine: a systematic review with economic modelling.慢性偏头痛成人的预防性药物治疗：系统评价与经济建模。
Health Technol Assess. 2024 Oct;28(63):1-329. doi: 10.3310/AYWA5297.
7
T cell and autoantibody recognition of nucleus-associated islet autoantigens in individuals with type 1 diabetes.1型糖尿病患者中T细胞和自身抗体对细胞核相关胰岛自身抗原的识别。
Diabetologia. 2025 Jun 9. doi: 10.1007/s00125-025-06458-8.
8
Systemic treatments for metastatic cutaneous melanoma.转移性皮肤黑色素瘤的全身治疗
Cochrane Database Syst Rev. 2018 Feb 6;2(2):CD011123. doi: 10.1002/14651858.CD011123.pub2.
9
A novel approach to hematopoietic stem cell transplantation: The effect of CMV infection and HLA genotypes on chimerism status in Egyptian patients.造血干细胞移植的一种新方法：巨细胞病毒感染和人类白细胞抗原基因型对埃及患者嵌合状态的影响。
Cell Immunol. 2025 Aug;414:104991. doi: 10.1016/j.cellimm.2025.104991. Epub 2025 Jun 7.
10
Allosensitisation in NHP results in cross-reactive anti-SLA antibodies not detected by a lymphocyte-based flow cytometry crossmatch.在非人类灵长类动物中，同种异体致敏会导致淋巴细胞交叉配型流式细胞术无法检测到的交叉反应性抗 SLA 抗体。
HLA. 2024 Jul;104(1):e15599. doi: 10.1111/tan.15599.
引用本文的文献
1
Differential interaction of magnetic nanoparticles with tumor cells and peripheral blood cells.磁性纳米颗粒与肿瘤细胞和外周血细胞的差异相互作用。
J Cancer Res Clin Oncol. 2006 May;132(5):287-92. doi: 10.1007/s00432-006-0076-x. Epub 2006 Jan 24.
2
Genetic and serological heterogeneity of the supertypic HLA-B locus specificities Bw4 and Bw6.超型HLA - B位点特异性Bw4和Bw6的遗传和血清学异质性。
Immunogenetics. 1989;30(3):200-7. doi: 10.1007/BF02421207.
3
Expression of an HLA-Bw6-related specificity by the HLA-Cw3 molecule.HLA - Cw3分子表达与HLA - Bw6相关的特异性。
Immunogenetics. 1985;21(5):469-78. doi: 10.1007/BF00430930.
4
Transfection of murine LMTK- cells with purified HLA class I genes. VII. Association of allele- and locus-specific serological reactivities with respectively the first and second domains of the HLA-B7 molecule.用纯化的HLA I类基因转染小鼠LMTK-细胞。VII. 等位基因和位点特异性血清学反应性分别与HLA - B7分子的第一和第二结构域的关联。
Immunogenetics. 1986;23(4):246-51. doi: 10.1007/BF00373019.
5
Differential regulation of HLA class I genes by interferon.干扰素对HLA I类基因的差异调节
Immunogenetics. 1990;31(4):245-52. doi: 10.1007/BF00204896.
本文引用的文献
1
Restitution potentials of allogeneically or xenogeneically grafted lymphocyte-free hemopoietic stem cells.
Haematol Blood Transfus. 1980;25:153-77. doi: 10.1007/978-3-642-67319-1_15.
2
Analysis of rat hemopoietic cells on the fluorescence-activated cell sorter. II. Isolation of terminal deoxynucleotidyl transferase-positive cells.用荧光激活细胞分选仪分析大鼠造血细胞。II. 末端脱氧核苷酸转移酶阳性细胞的分离。
J Exp Med. 1980 Aug 1;152(2):438-46. doi: 10.1084/jem.152.2.438.
3
Analysis of rat hemopoietic cells on the fluorescence-activated cell sorter. I. Isolation of pluripotent hemopoietic stem cells and granulocyte-macrophage progenitor cells.利用荧光激活细胞分选仪分析大鼠造血细胞。I. 多能造血干细胞和粒细胞-巨噬细胞祖细胞的分离。
J Exp Med. 1980 Aug 1;152(2):419-37. doi: 10.1084/jem.152.2.419.
4
The separation of cell populations using monoclonal antibodies attached to sepharose.使用附着于琼脂糖的单克隆抗体分离细胞群体。
Tissue Antigens. 1980 Oct;16(4):317-25. doi: 10.1111/j.1399-0039.1980.tb00313.x.
5
Helix pomatia agglutinin (HpA) affinity chromatography: the isolation of pure B and T cell populations and their use for the routine HLA-DR (Ia) serology.罗马蜗牛凝集素（HpA）亲和层析法：纯B细胞和T细胞群体的分离及其在常规HLA-DR（Ia）血清学中的应用。
J Immunol Methods. 1980;32(3):285-96. doi: 10.1016/0022-1759(80)90193-3.
6
Separation of human bone marrow cells in density gradients of polyvinylpyrrolidone coated silica gel (Percoll).在聚乙烯吡咯烷酮包被硅胶（Percoll）密度梯度中分离人骨髓细胞。
Scand J Haematol. 1980 Mar;24(3):254-62. doi: 10.1111/j.1600-0609.1980.tb01334.x.
7
Separation of human blood monocytes and lymphocytes on a continuous Percoll gradient.在连续的Percoll梯度上分离人血单核细胞和淋巴细胞。
J Immunol Methods. 1980;33(1):1-9. doi: 10.1016/0022-1759(80)90077-0.
8
Free flow electrophoretic separation of T and B lymphocytes. Evidence for various subpopulations of B cells.T 淋巴细胞和 B 淋巴细胞的自由流动电泳分离。B 细胞不同亚群的证据。
Hoppe Seylers Z Physiol Chem. 1972 Jan;353(1):105-10.
9
Fluorescence activated cell sorting.荧光激活细胞分选
Rev Sci Instrum. 1972 Mar;43(3):404-9. doi: 10.1063/1.1685647.
10
Specific fractionation of immunocompetent cells.免疫活性细胞的特异性分级分离
Transplant Rev. 1970;5:76-104. doi: 10.1111/j.1600-065x.1970.tb00357.x.
Zotero 插件