1-O-烷基甘油基3-磷酸胆碱（O-脱乙酰基血小板活化因子）从白细胞中的释放：磷脂的化学电离质谱分析
Release of 1-O-alkylglyceryl 3-phosphorylcholine, O-deacetyl platelet-activating factor, from leukocytes: chemical ionization mass spectrometry of phospholipids.
作者信息
Polonsky J, Tencé M, Varenne P, Das B C, Lunel J, Benveniste J
出版信息
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7019-23. doi: 10.1073/pnas.77.12.7019.
Abstract
Evidence is presented for the simultaneous release of platelet-activating factor (PAF-acether) and of its deacetylated derivative (lyso-PAF-acether) from hog leukocytes. On the basis of spectroscopy and chemical reactions, the structure of O-deacetyl-PAF is shown to be 1-O-alkylglyceryl 3-phosphorylcholine, an alkyl ether analog of lyso-phosphatidylcholine. Acetylation of lyso-PAF yields a compound with biological activity and chromatographical behavior indistinguishable from those of native PAF. Lyso-PAF may be considered to be either the precursor or the enzymatic degradation product of PAF. The usefulness of chemical ionization mass spectrometry for structural determination of phospholipids is also demonstrated.
摘要
有证据表明，猪白细胞能同时释放血小板活化因子（PAF-乙醚）及其脱乙酰化衍生物（溶血-PAF-乙醚）。基于光谱学和化学反应，O-脱乙酰基-PAF的结构被证明为1-O-烷基甘油基3-磷酸胆碱，即溶血磷脂酰胆碱的烷基醚类似物。溶血-PAF的乙酰化产生一种生物活性和色谱行为与天然PAF无法区分的化合物。溶血-PAF可被认为是PAF的前体或酶促降解产物。还证明了化学电离质谱法在磷脂结构测定中的实用性。
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