通过DNA和染色质介导的基因转移导入小鼠LMTK-细胞的胸苷激酶活性的细胞周期依赖性调控。
Cell cycle-dependent regulation of thymidine kinase activity introduced into mouse LMTK- cells by DNA and chromatin-mediated gene transfer.
作者信息
Schlosser C A, Steglich C, deWet J R, Scheffler I E
出版信息
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1119-23. doi: 10.1073/pnas.78.2.1119.
Abstract
We report on the expression of thymidine kinase (tk) activity in synchronized populations of mouse cells that have been transformed to the tk+ phenotype with purified DNA from various sources or with metaphase chromosomes from human cells. The viral (herpes) tk gene is constitutively expressed but, in all other cases examined, the activity is regulated as in normal tk+ mouse cells: There is a dramatic increase at the beginning of the S-phase. This regulation is observed whether the transgenome is stably integrated into the host genome or whether it is still in an unstable nonintegrated state.
摘要
我们报告了在已用来自各种来源的纯化DNA或来自人类细胞的中期染色体转化为tk+表型的小鼠细胞同步群体中胸苷激酶（tk）活性的表达情况。病毒（疱疹）tk基因是组成型表达的，但在所有其他检测的情况下，其活性与正常tk+小鼠细胞一样受到调节：在S期开始时会急剧增加。无论转基因基因组是稳定整合到宿主基因组中，还是仍处于不稳定的非整合状态，都能观察到这种调节。
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本文引用的文献
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Expression of human genes for adenine phosphoribosyltransferase and hypoxanthine-guanine phosphoribosyltransferase after genetic transformation of mouse cells with purified human DNA.用纯化的人类DNA对小鼠细胞进行基因转化后腺嘌呤磷酸核糖基转移酶和次黄嘌呤-鸟嘌呤磷酸核糖基转移酶的人类基因表达
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