稳定的小鼠胸苷激酶mRNA的细胞周期调控合成是由cDNA中的一个序列介导的。
Cell cycle regulated synthesis of stable mouse thymidine kinase mRNA is mediated by a sequence within the cDNA.
作者信息
Hofbauer R, Müllner E, Seiser C, Wintersberger E
出版信息
Nucleic Acids Res. 1987 Jan 26;15(2):741-52. doi: 10.1093/nar/15.2.741.
The cDNA for mouse thymidine kinase (TK) was isolated from a cDNA library in lambda-gt11 and sequenced. It was used as a probe to follow the time course of TK mRNA expression in growth stimulated mouse fibroblasts. Linked to the HSV-TK promoter the cDNA was able to transform LTK-cells to the TK+ phenotype. The transformed cells expressed the TK mRNA and enzyme activity in a growth dependent fashion suggesting that the regulatory element is localized on the cDNA.
从小鼠λ-gt11 cDNA文库中分离出小鼠胸苷激酶（TK）的cDNA并进行测序。用它作为探针追踪生长刺激的小鼠成纤维细胞中TK mRNA表达的时间进程。与单纯疱疹病毒胸苷激酶（HSV-TK）启动子相连的cDNA能够将LTK⁻细胞转化为TK⁺表型。转化细胞以生长依赖的方式表达TK mRNA和酶活性，这表明调控元件定位于该cDNA上。
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