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鸡tk基因的遗传与物理分析。

Genetic and physical analysis of the chicken tk gene.

作者信息

Merrill G F, Harland R M, Groudine M, McKnight S L

出版信息

Mol Cell Biol. 1984 Sep;4(9):1769-76. doi: 10.1128/mcb.4.9.1769-1776.1984.

DOI:10.1128/mcb.4.9.1769-1776.1984
PMID:6092937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC368985/
Abstract

Several aspects of the structure of the chicken thymidine kinase gene (tk) have been resolved as a result of genetic experiments and nucleotide sequencing. Deletion mapping established the locations of two functional boundaries in a region thought to correspond to the 5' terminus of the gene. One such boundary coincides with a transcriptional promoter, and the other coincides with the translation start codon of the chicken tk polypeptide. Similar deletion mapping assays identified a functional boundary at the 3' terminus of the gene. DNA sequence analysis confirms the prediction that this 3' region encodes the carboxyl terminus of the tk polypeptide. A recombinant cDNA clone complementary to genomic tk sequences was isolated. A comparison between genomic and cDNA sequences reveals the locations of six intervening sequences and allows prediction of the complete amino acid sequence of the chicken tk polypeptide.

摘要

通过基因实验和核苷酸测序，鸡胸苷激酶基因（tk）结构的几个方面已得到解析。缺失图谱确定了一个被认为对应于该基因5'末端区域内两个功能边界的位置。其中一个这样的边界与转录启动子重合，另一个与鸡tk多肽的翻译起始密码子重合。类似的缺失图谱分析在该基因的3'末端鉴定出一个功能边界。DNA序列分析证实了这一预测，即该3'区域编码tk多肽的羧基末端。分离出了一个与基因组tk序列互补的重组cDNA克隆。基因组序列与cDNA序列的比较揭示了六个间隔序列的位置，并使得能够预测鸡tk多肽的完整氨基酸序列。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ef9/368985/c4747bb7d3d5/molcellb00151-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ef9/368985/c4747bb7d3d5/molcellb00151-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6ef9/368985/c4747bb7d3d5/molcellb00151-0111-a.jpg

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本文引用的文献

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J Biol Chem. 1980 Aug 10;255(15):7386-90.
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Enhancing the efficiency of DNA-mediated gene transfer in mammalian cells.提高DNA介导的基因转移在哺乳动物细胞中的效率。
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Mol Cell Biol. 1985 Jul;5(7):1694-706. doi: 10.1128/mcb.5.7.1694-1706.1985.
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Adenosine deaminase: characterization and expression of a gene with a remarkable promoter.腺苷脱氨酶：一个具有独特启动子的基因的特性与表达
EMBO J. 1985 Feb;4(2):437-43. doi: 10.1002/j.1460-2075.1985.tb03648.x.
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