Koskinen K P, Rahkamo A, Tuompo H
Scand J Dent Res. 1981 Feb;89(1):71-8. doi: 10.1111/j.1600-0722.1981.tb01279.x.
The cytotoxicity of seven solutions used in root canal therapy was tested in human fibroblast and lymphoblast cultures. The amount of cell damage was assessed by measuring the release of 51Cr from labeled cells into the medium. The solutions, when applied at therapeutic concentrations, displayed high toxicity in vitro and differences in cytotoxicity were seen between different solutions. Generally, lymphoblasts were found to be more sensitive than fibroblasts. The cytotoxic profiles of the two cell types resembled each other except when 5% sodium hypochlorite or 0.2% Hibitane was used. When the criterion of total cell lysis was 50% 51Cr release, the toxic concentrations of the solutions tested ranged between 1:25 and 1:900 (v/v) for fibroblasts. For lymphoblasts the corresponding concentration range was between 1:40 and 1:750. Despite technical simplicity and good reproducibility the 51Cr release method proved unreliable for testing the cytotoxicity of endodontic solutions. Because the methodological errors cannot be foreseen the 51Cr release method requires supporting evidence from other methods.
在人成纤维细胞和淋巴细胞培养物中测试了用于根管治疗的七种溶液的细胞毒性。通过测量标记细胞中51Cr释放到培养基中的量来评估细胞损伤程度。这些溶液在治疗浓度下应用时，在体外显示出高毒性，并且不同溶液之间存在细胞毒性差异。一般来说，发现淋巴细胞比成纤维细胞更敏感。除了使用5%次氯酸钠或0.2%氯己定时，两种细胞类型的细胞毒性谱彼此相似。当成纤维细胞完全细胞裂解的标准为51Cr释放50%时，测试溶液的毒性浓度范围为1:25至1:900（v/v）。对于淋巴细胞，相应的浓度范围为1:40至1:750。尽管51Cr释放方法技术简单且重现性好，但事实证明它对于测试牙髓治疗溶液的细胞毒性并不可靠。由于无法预见方法学误差，51Cr释放方法需要其他方法的支持证据。
