Cotti Elisabetta, Petreucic Vittorio, Re Dino, Simbula Gabriella
Department of Conservative Dentistry and Endodontics, School of Dentistry, University of Cagliari, Cagliari, Italy.
Department of Conservative Dentistry and Endodontics, School of Dentistry, University of Cagliari, Cagliari, Italy.
J Endod. 2014 Jan;40(1):124-8. doi: 10.1016/j.joen.2013.09.038. Epub 2013 Oct 31.
This study evaluated the cytotoxicity of the new experimental self-adhesive, methachrylate-based hybrid root canal sealer XT and compared it with the epoxy resin-based AH Plus Jet.
The cytotoxicity of the tested materials was evaluated after 1, 24, 48, and 72 hours by using growing and confluent mouse fibroblast cell line L929. L929 fibroblasts were maintained in Dulbecco modified medium containing 10% fetal calf serum at 37°C and 5% CO2. At confluence, cells were seeded in 24-well plates at concentration of 1.5 × 10(5) cells (growing cells) or 2.5 × 10(5) (confluent cells) for each well. An amount of 5 μL of each root sealer (mixed according to manufacturer's specifications) was placed into individual wells containing a monolayer of L929 cells to mimic the in vivo condition of the possible extrusion of sealer in the periapical tissues. Neutral Red and [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide] were used for the cytotoxicity evaluation. Untreated cells were used as control. Two-way analysis of variance with Bonferroni test was used to compare the toxicity of the 2 sealers; one-way analysis of variance with Tukey test was performed to compare the cytotoxicity of each sealer at any considered time points (P < .05). Results were confirmed by examination with optical microscope.
Both sealers induced a time-dependent cell death of mouse fibroblast L929; however, XT was less cytotoxic than AH Plus Jet as indicated by viability and morphologic analyses, and its initial cytotoxicity decreased progressively over time.
These data support the possible use of XT as an endodontic sealer.
本研究评估了新型实验性自粘型甲基丙烯酸酯基混合根管封闭剂XT的细胞毒性，并将其与环氧树脂基AH Plus Jet进行比较。
使用生长状态和融合状态的小鼠成纤维细胞系L929，在1、24、48和72小时后评估受试材料的细胞毒性。L929成纤维细胞在含10%胎牛血清的杜尔贝科改良培养基中，于37°C和5%二氧化碳条件下培养。细胞融合时，以每孔1.5×10⁵个细胞（生长状态细胞）或2.5×10⁵个细胞（融合状态细胞）的浓度接种于24孔板。将5μL每种根管封闭剂（按制造商规格混合）置于含有单层L929细胞的各个孔中，以模拟封闭剂在根尖周组织中可能挤出的体内情况。使用中性红和[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑]进行细胞毒性评估。未处理的细胞用作对照。采用带有邦费罗尼检验的双向方差分析来比较两种封闭剂的毒性；采用带有图基检验的单向方差分析来比较每种封闭剂在任何考虑的时间点的细胞毒性（P <.05）。结果通过光学显微镜检查得到证实。
两种封闭剂均诱导小鼠成纤维细胞L929出现时间依赖性细胞死亡；然而，从活力和形态学分析来看，XT的细胞毒性低于AH Plus Jet，并且其初始细胞毒性随时间逐渐降低。
这些数据支持将XT用作根管封闭剂的可能性。
