Vajrabhaya L, Sithisarn P, Wilairat P, Leelaphiwat S
Department of Operative Dentistry, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
J Endod. 1997 Jun;23(6):355-7. doi: 10.1016/s0099-2399(97)80181-1.
The aim of this study was to evaluate Sulphorhodamine-B (SRB) staining against 51Cr-release in cytotoxicity tests of six endodontic sealers, namely, MU sealer (Mahidol University) ROCANAL 2, ROCANAL 3, Apexit, Endomethasone, and AH-26. Monolayers (5 x 10(5) cells/ml) of the mouse cell line Mu-mu-1 were used as test cells. Following incubation at 37 degrees C in 5% CO2 for 24 h in the presence of each sealer, cells were stained with 0.4% SRB and the absorbance at 540 nm determined as measure of cell viability. For 51Cr-release assay, cells were labelled with 51Cr before testing with sealers, and radioactivity in the supernatant was measured in a liquid scintillation counter. Both techniques indicated that Apexit was the least toxic sealer. In view of the ease of conducting SRB staining for tests of cell viability, this may be the method of choice over 51Cr-release assay in the evaluation of endodontic sealer cytotoxicity.
本研究的目的是在六种根管封闭剂（即MU封闭剂（玛希隆大学）、ROCANAL 2、ROCANAL 3、Apexit、Endomethasone和AH - 26）的细胞毒性试验中，评估磺酰罗丹明 - B（SRB）染色法与51铬释放法的效果。小鼠细胞系Mu - mu - 1的单层细胞（5×10⁵细胞/毫升）用作测试细胞。在每种封闭剂存在的情况下，于37℃、5%二氧化碳条件下孵育24小时后，细胞用0.4%的SRB染色，并测定540纳米处的吸光度作为细胞活力的指标。对于51铬释放试验，在使用封闭剂测试前，细胞用51铬标记，然后用液体闪烁计数器测量上清液中的放射性。两种技术均表明Apexit是毒性最小的封闭剂。鉴于进行SRB染色以测试细胞活力操作简便，在评估根管封闭剂的细胞毒性时，这可能是优于51铬释放试验的首选方法。
