Dutta S K, Gorgacz E J, Albert T F, Ingling A L
Am J Vet Res. 1977 May;38(5):591-5.
A subcutaneous neoplastic mass in a 13-lined ground squirrel which metastasized to regional lymph nodes and lung was studied. Histopathologically, the tumor architecture and cellular morphology were compatible with that of a malignant amelanotic melanoma. Ultrastructurally, the neoplastic tissue was composed of oval cells, spindle-shaped cells, and spindle-shaped cells with electron-dense cytoplasmic granules. Virus particles were not seen in these cells. Cell cultures from neoplastic tissue grew in complete monolayers and on initial passages contained a few herpesvirus particles. Secondary monolayer cell culture, when exposed to 5-iodo-2'-deoxyuridine or made into several serial subculture passages, caused the appearance of cytopathic effect and the demonstration of many virus particles. The ground squirrel agent, because it contained DNA, was sensitive to chloroform treated and had herpesvirus characteristics on electron microscopy, was considered a herpesvirus. The buoyant density of the virus was 1.298 g/cm3 and the diameter of the enveloped virus particles was 146 nm. This ground squirrel herpesvirus was antigenically distinct from other known herpesviruses.
对一只13条纹地松鼠身上的皮下肿瘤块进行了研究，该肿瘤已转移至局部淋巴结和肺部。组织病理学上，肿瘤结构和细胞形态与恶性无色素性黑色素瘤相符。超微结构上，肿瘤组织由椭圆形细胞、梭形细胞以及带有电子致密细胞质颗粒的梭形细胞组成。在这些细胞中未发现病毒颗粒。肿瘤组织的细胞培养物能形成完整的单层，初代传代培养时含有少量疱疹病毒颗粒。二代单层细胞培养物在接触5-碘-2'-脱氧尿苷或进行多次连续传代培养后，出现细胞病变效应并显示出许多病毒颗粒。该松鼠病原体因含有DNA，对氯仿处理敏感且在电子显微镜下具有疱疹病毒特征，被认为是一种疱疹病毒。该病毒的浮力密度为1.298 g/cm³，包膜病毒颗粒直径为146 nm。这种松鼠疱疹病毒在抗原性上与其他已知疱疹病毒不同。
