High performance liquid chromatographic determination of aclacinomycin A and its related compounds. I. Normal phase HPLC for monitoring fermentation and purification processes.

A normal phase high performance liquid chromatographic method is presented for the separation and quantitative determination of aclacinomycin A (ACM) and its related compounds classified as anthracycline antibiotics. The sensitive, reliable and reproducible chromatography was achieved on a mu-Porasil column with a mobile phase consisting of chloroform-methanol-acetic acid-water-triethylamine (68:20:10:2:0.01, v/v). By use of MA144 M1, one of the ACM analogues, as an internal standard, selectivity of the HPLC method could be retained by virtue of the adjustment of the detecting wavelength to 436 nm. The HPLC method was applicable to monitoring the fermentation and purification processes.