Laskaris G
Oral Surg Oral Med Oral Pathol. 1981 Jun;51(6):626-31. doi: 10.1016/s0030-4220(81)80013-8.
Oral mucosa biopsy specimens and sera from fifty-eight patients with pemphigus vulgaris limited to the oral cavity were studied by the direct and indirect immunofluorescence technique. Direct immunofluorescence demonstrated intercellular substance (ICS) deposition of IgG, either alone or in combination with C3, IgA, and IgM, in fifty-seven out of fifty-eight (98.3 per cent) oral mucosa biopsy specimens examined. At the time of initial evaluation circulating ICS antibodies were detected in twenty-eight out of fifty-eight patients (48.3 per cent) when a compound animal epithelial tissue was used as substrate. However, when normal human oral mucosa was used as substrate, circulating ICS antibodies were observed in fifty out of fifty-eight patients (86.2 per cent). It is concluded that direct and indirect immunofluorescence is a valuable diagnostic test in the early diagnosis of oral pemphigus vulgaris. In addition, normal human oral mucosa is a more appropriate substrate than animal epithelial tissue.
采用直接免疫荧光和间接免疫荧光技术，对58例局限于口腔的寻常型天疱疮患者的口腔黏膜活检标本和血清进行了研究。在检查的58份口腔黏膜活检标本中，57份（98.3%）通过直接免疫荧光显示细胞间物质（ICS）有IgG沉积，单独或与C3、IgA和IgM联合沉积。在初始评估时，当使用复合动物上皮组织作为底物时，58例患者中有28例（48.3%）检测到循环ICS抗体。然而，当使用正常人口腔黏膜作为底物时，58例患者中有50例（86.2%）观察到循环ICS抗体。结论是，直接免疫荧光和间接免疫荧光在口腔寻常型天疱疮的早期诊断中是一种有价值的诊断试验。此外，正常人口腔黏膜比动物上皮组织是更合适的底物。
