Laskaris G, Papanicolaou S, Angelopoulos A
Oral Surg Oral Med Oral Pathol. 1981 May;51(5):531-4. doi: 10.1016/0030-4220(81)90015-3.
This study was undertaken in order to explore the diagnostic efficacy of the direct immunofluorescent technique for demonstration in vivo bound immunoglobulins to desquamated epithelial cells from patients with oral pemphigus. Cytologic smears were obtained with a wooden spatula from the oral lesions of thirty patients with known pemphigus vulgaris. Control smears were taken from thirty-five patients twenty suffering from various other mucosal diseases and fifteen devoid of oral lesions. The smears were stored at -30 degrees C. and prepared within 48 hours for examination by the direct immunofluorescent technique. Deposition of IgG was demonstrated in all thirty smears from the oral pemphigus lesions. None of the control smears gave positive results. These findings suggest that the direct immunofluorescent technique using cytologic smears may provide an additional simple and reliable method in the diagnosis or oral pemphigus vulgaris.
本研究旨在探讨直接免疫荧光技术对口腔天疱疮患者脱屑上皮细胞中体内结合免疫球蛋白的诊断效能。用木刮刀从30例已知寻常型天疱疮患者的口腔病变处获取细胞学涂片。对照涂片取自35例患者，其中20例患有各种其他黏膜疾病，15例无口腔病变。涂片保存在-30℃，并在48小时内制备好，用直接免疫荧光技术进行检查。在所有30例口腔天疱疮病变的涂片中均显示有IgG沉积。对照涂片均未得出阳性结果。这些发现表明，使用细胞学涂片的直接免疫荧光技术可能为寻常型口腔天疱疮的诊断提供一种额外的简单可靠方法。
