Bode U, Deisseroth A, Hendrick D
Proc Natl Acad Sci U S A. 1981 May;78(5):2815-9. doi: 10.1073/pnas.78.5.2815.
The nonhistone chromosomal proteins of a series of hybrid mouse erythroleukemia cell lines containing human chromosome 16 were investigated by two-dimensional gel electrophoresis to determine if such cells contained nonhistone chromosomal proteins of both human and mouse origin. Comparison of the two-dimensional gel electrophoretograms of the nonhistone chromosomal proteins of mouse and human cell lines showed 400 and 280 chromosomal proteins, respectively, of which about 75% were electrophoretically identical. The two-dimensional gel electrophoretogram of a cloned hybrid mouse erythroleukemia cell line that retained a tetraploid complement of mouse chromosomes and human chromosome 16 (as the only human chromosome) displayed a nonhistone chromosomal protein of pI 6.2 and Mr 65,000. This protein, which comigrates with a nonhistone chromosomal protein present in the human cell line used to produce this hybrid cell and which is also present in two additional human cells lines studied, could not be detected in the mouse erythroleukemia parent before fusion. This polypeptide also was shown by similar techniques to be associated with the presence of human chromosome 16 in four out of five other independently derived hybrid mouse erythroleukemia cell lines that contained a near tetraploid complement of mouse erythroleukemia chromosomes.
通过二维凝胶电泳对一系列含有人类16号染色体的杂交小鼠红白血病细胞系的非组蛋白染色体蛋白质进行了研究，以确定这些细胞是否含有源自人类和小鼠的非组蛋白染色体蛋白质。小鼠和人类细胞系非组蛋白染色体蛋白质的二维凝胶电泳图谱比较显示，分别有400种和280种染色体蛋白质，其中约75%在电泳上是相同的。一个克隆的杂交小鼠红白血病细胞系保留了小鼠染色体的四倍体补体和人类16号染色体（作为唯一的人类染色体），其二维凝胶电泳图谱显示有一个等电点为6.2、分子量为65,000的非组蛋白染色体蛋白质。这种蛋白质与用于产生该杂交细胞的人类细胞系中存在的一种非组蛋白染色体蛋白质迁移率相同，并且在另外两个研究的人类细胞系中也存在，在融合前的小鼠红白血病亲本中未检测到。通过类似技术还表明，在其他五个独立衍生的杂交小鼠红白血病细胞系中，有四个含有接近四倍体补体的小鼠红白血病染色体，这种多肽与人类16号染色体的存在有关。
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