功能性人糖皮质激素受体cDNA的一级结构与表达
Primary structure and expression of a functional human glucocorticoid receptor cDNA.
作者信息
Hollenberg S M, Weinberger C, Ong E S, Cerelli G, Oro A, Lebo R, Thompson E B, Rosenfeld M G, Evans R M
出版信息
Nature. 1985 Dec 19;318(6047):635-41. doi: 10.1038/318635a0.
Abstract
Identification of complementary DNAs encoding the human glucocorticoid receptor predicts two protein forms, of 777 (alpha) and 742 (beta) amino acids, which differ at their carboxy termini. The proteins contain a cysteine/lysine/arginine-rich region which may define the DNA-binding domain. Pure radiolabelled glucocorticoid receptor, synthesized in vitro, is immunoreactive and possesses intrinsic steroid-binding activity characteristic of the native glucocorticoid receptor.
摘要
编码人类糖皮质激素受体的互补DNA的鉴定预测出两种蛋白质形式，分别由777个（α）和742个（β）氨基酸组成，它们在羧基末端有所不同。这些蛋白质含有一个富含半胱氨酸/赖氨酸/精氨酸的区域，该区域可能界定了DNA结合结构域。体外合成的纯放射性标记糖皮质激素受体具有免疫反应性，并具有天然糖皮质激素受体特有的内在类固醇结合活性。
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本文引用的文献
1
Metallothionein mRNA induction in HeLa cells in response to zinc or dexamethasone is a primary induction response.HeLa细胞中金属硫蛋白mRNA对锌或地塞米松的诱导是一种初级诱导反应。
Nature. 1980 Jul 17;286(5770):295-7. doi: 10.1038/286295a0.
2
Characterization of an antiserum against the glucocorticoid receptor.一种抗糖皮质激素受体抗血清的特性鉴定。
Biochim Biophys Acta. 1981 Oct 12;677(2):205-19. doi: 10.1016/0304-4165(81)90087-8.
3
Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoids.糖皮质激素对小鼠肝脏金属硫蛋白-I基因的转录调控
Nature. 1981 May 28;291(5813):340-2. doi: 10.1038/291340a0.
4
Monoclonal antibodies to the rat liver glucocorticoid receptor.针对大鼠肝脏糖皮质激素受体的单克隆抗体。
EMBO J. 1982;1(11):1467-71. doi: 10.1002/j.1460-2075.1982.tb01339.x.
5
Active domains in wild-type and mutant glucocorticoid receptors.野生型和突变型糖皮质激素受体中的活性结构域。
EMBO J. 1982;1(3):285-9. doi: 10.1002/j.1460-2075.1982.tb01161.x.
6
Activation of vitellogenin gene transcription is a direct response to estrogen in Xenopus laevis liver.在非洲爪蟾肝脏中，卵黄蛋白原基因转录的激活是对雌激素的直接反应。
Nucleic Acids Res. 1982 Dec 20;10(24):8273-84. doi: 10.1093/nar/10.24.8273.
7
Growth hormone gene transcription is regulated by thyroid and glucocorticoid hormones in cultured rat pituitary tumor cells.在培养的大鼠垂体肿瘤细胞中，生长激素基因转录受甲状腺激素和糖皮质激素的调节。
J Biol Chem. 1982 Oct 10;257(19):11627-32.
8
Regulated expression of human growth hormone genes in mouse cells.人生长激素基因在小鼠细胞中的调控表达。
Cell. 1982 Jun;29(2):623-31. doi: 10.1016/0092-8674(82)90178-7.
9
Dexamethasone 21-mesylate: an affinity label of glucocorticoid receptors from rat hepatoma tissue culture cells.地塞米松21-甲磺酸盐：大鼠肝癌组织培养细胞糖皮质激素受体的亲和标记物。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3541-5. doi: 10.1073/pnas.78.6.3541.
10
Expression of human and mouse nonhistone chromosomal proteins in hybrid mouse erythroleukemia cells containing a single human chromosome.在含有一条人类染色体的杂交小鼠红白血病细胞中人类和小鼠非组蛋白染色体蛋白的表达
Proc Natl Acad Sci U S A. 1981 May;78(5):2815-9. doi: 10.1073/pnas.78.5.2815.
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