染色体蛋白的一部分对体外mRNA转录的调控。
Regulation of in vitro mRNA transcription by a fraction of chromosomal proteins.
作者信息
Chiu J F, Tsai Y H, Sakuma K, Hnilica L S
出版信息
J Biol Chem. 1975 Dec 25;250(24):9431-3.
PMID:1194293
Abstract
A fractionation scheme was developed which permits the isolation of chromosomal non-histone protein fraction associated with DNA in chromatin. This fraction which represents less than 10% of the total protein content of reticulocyte chromatin was found to be essential for the in vitro transcription of globin mRNA by chromatin preparations reconstituted from DNA and isolated chromosomal protein components.
摘要
已开发出一种分级分离方案，该方案可分离与染色质中DNA相关的染色体非组蛋白部分。发现这一部分占网织红细胞染色质总蛋白含量不到10%，对于由DNA和分离的染色体蛋白成分重构的染色质制剂在体外转录珠蛋白mRNA至关重要。
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