Nehlig A, Lehr P R
Brain Res. 1981 Aug;254(1):89-96. doi: 10.1016/0165-3806(81)90061-4.
The 3-oxo acid-CoA transferase and acetoacetyl-CoA synthetase activities were estimated in subcellular fractions isolated from the cerebral hemispheres, the optic lobes, the cerebellum and the liver of the chick between the twentieth day of embryonic life and the thirtieth day of postnatal development. In the 3 areas of chick brain, the transferase activity increases during the whole postnatal development. Transferase is located both in mitochondria and microsomes unlike in the rat brain where this enzyme is specifically mitochondrial. There is no detectable activity of synthetase in the chick brain. These differences in enzyme localization imply that acetoacetate is converted into acetoacetyl-CoA only by 3-oxo acid-CoA transferase in the mitochondria and cytoplasm of the chick brain, whereas, in the rat brain, this reaction is performed by 3-oxo acid-CoA transferase in mitochondria and by acetoacetyl-CoA synthetase in the cytosol. There is no detectable activity either for transferase or for synthetase in the chick liver.
在胚胎期第20天至出生后第30天的雏鸡中，对从大脑半球、视叶、小脑和肝脏分离出的亚细胞组分中的3-氧代酸-CoA转移酶和乙酰乙酰-CoA合成酶活性进行了评估。在雏鸡大脑的3个区域中，转移酶活性在整个出生后发育过程中均增加。与大鼠脑不同，大鼠脑中该酶特异性定位于线粒体，而在雏鸡脑中，转移酶既存在于线粒体中，也存在于微粒体中。在雏鸡脑中未检测到合成酶活性。这些酶定位的差异表明，在雏鸡脑的线粒体和细胞质中，乙酰乙酸仅通过3-氧代酸-CoA转移酶转化为乙酰乙酰-CoA，而在大鼠脑中，该反应由线粒体中的3-氧代酸-CoA转移酶和胞质溶胶中的乙酰乙酰-CoA合成酶进行。在雏鸡肝脏中，转移酶和合成酶均未检测到活性。
