Zawilska K, Komarnicki M, Trepińska E, Sokołowski K, Wysocki K
Haemostasis. 1981;10(5):233-44. doi: 10.1159/000214407.
The effect of blastic cells isolated by Böyum's method from the blood of 20 nontreated patients with acute myelogenous leukaemia on normal platelet aggregation has been studied. The isolated blasts in concentration of 2,000/microliter and higher, inhibit normal platelet aggregation induced by ADP or collagen, but have no influence on aggregation initiated by epinephrine. This effect is unaffected by the presence of metabolic poisons or by the disruption of the blastic cell membrane. Our results seem to exclude interference of blastic cells with platelet prostaglandin biosynthesis. In patients with acute leukaemia and an increased leukocyte count the preparation of platelet suspension free of blastic cells is not possible, even by the albumin gradient method or by gel filtration. The leukaemic blast content in platelet-rich suspensions may account for the variations in the aggregation behaviour which are found in these patients. The influence of blastic cells may be an important factor in the bleeding tendency in myelogenous leukaemia
研究了用博伊姆方法从20例未经治疗的急性髓性白血病患者血液中分离出的原始细胞对正常血小板聚集的影响。浓度为2000/微升及以上的分离原始细胞可抑制由ADP或胶原诱导的正常血小板聚集，但对肾上腺素引发的聚集没有影响。这种作用不受代谢毒物的存在或原始细胞膜破坏的影响。我们的结果似乎排除了原始细胞对血小板前列腺素生物合成的干扰。在急性白血病且白细胞计数增加的患者中，即使采用白蛋白梯度法或凝胶过滤法，也无法制备不含原始细胞的血小板悬液。富含血小板的悬液中的白血病原始细胞含量可能是这些患者中发现的聚集行为变化的原因。原始细胞的影响可能是髓性白血病出血倾向的一个重要因素。
