Sugiyama T, Ueda N, Maeda S, Shiraishi N, Goto-Mimura K, Murao S, Chattopadhyay S C
J Natl Cancer Inst. 1981 Oct;67(4):831-9.
The chromosomal distribution of chromosome aberrations (CA) and sister chromatid exchanges (SCE) induced by various chemical carcinogens such as 7,12-dimethylbenz[a]anthracene, 7,8,12-trimethylbenz[a]anthracene, 1-butyl-1-nitrosourea, urethan, 4-nitroquinoline 1-oxide, 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, and the antineoplastic compound mitomycin C was studied with the use of noninbred Long-Evans male rat bone marrow cells in vivo and in vitro. The CA and SCE induced by these structurally different chemicals were distributed in a similar pattern among and along chromosomes when the chemicals were given a short time (6 hr) before the metaphase cells were harvested. The specific distribution pattern of chemically induced CA and SCE along chromosomes was attributed to the late DNA replication of the vulnerable chromosome regions. Conversely, X-ray-induced CA were distributed more randomly. Thus the different chemical carcinogens were shown to exert a similar genetic effect at the chromosome level.
利用非近交系雄性长-伊文斯大鼠骨髓细胞，在体内和体外研究了多种化学致癌物（如7,12-二甲基苯并[a]蒽、7,8,12-三甲基苯并[a]蒽、1-丁基-1-亚硝基脲、尿烷、4-硝基喹啉1-氧化物、2-(2-呋喃基)-3-(5-硝基-2-呋喃基)丙烯酰胺）以及抗肿瘤化合物丝裂霉素C诱导的染色体畸变（CA）和姐妹染色单体交换（SCE）的染色体分布情况。当在中期细胞收获前短时间（6小时）给予这些化学物质时，由这些结构不同的化学物质诱导的CA和SCE在染色体之间以及沿染色体呈现相似的分布模式。化学诱导的CA和SCE沿染色体的特定分布模式归因于易损染色体区域的DNA晚期复制。相反，X射线诱导的CA分布更随机。因此，不同的化学致癌物在染色体水平上表现出相似的遗传效应。
