Banerjee R, Goldfeder A, Mitra J
J Natl Cancer Inst. 1983 Mar;70(3):517-21.
The frequency of sister chromatid exchanges (SCE) in vivo and chromosome aberrations and/or alterations were analyzed from the bone marrow cells of the treated dbrB tumor-bearing DBA/1J inbred mouse host. The results were compared with analogous data obtained from the bone marrow cells of untreated tumor-bearing mice for evaluation of the "indirect," i.e., somatic stress, effect on the normal host cells following triple-agent therapy intended for a mammary adenocarcinoma. Misonidazole (MIS), which is a known radiosensitizing drug, microwave hyperthermia (delta), and X-radiation (X) were used as therapeutic agents. Significant (P less than 0.05) numbers of SCE were induced in the bone marrow cells of the mice whose tumors received these triple-agent treatments (MIS + delta + X) simultaneously as compared with values of SCE per cell noted in bone marrow cells of untreated tumor-bearing control mice. The highest number of chromosome aberrations and alterations, including an increase in heteroploidy, was also noticed in the bone marrow cells of the mice whose tumors were treated simultaneously with MIS + delta + X. The triple-agent therapy on dbrB tumor also resulted in an unusually high polyploid metaphase plate in the bone marrow cell consisting of 320 chromosomes, indicating that this mode of therapy may act directly on the genetic material of the tumor-bearing host cells, inducing cytogenetic abnormalities as a side effect.
对经治疗的携带dbrB肿瘤的DBA/1J近交系小鼠宿主的骨髓细胞，分析了体内姐妹染色单体交换（SCE）的频率以及染色体畸变和/或改变情况。将结果与从未经治疗的荷瘤小鼠骨髓细胞获得的类似数据进行比较，以评估针对乳腺腺癌的三联疗法对正常宿主细胞的“间接”即躯体应激效应。已知的放射增敏药物米索硝唑（MIS）、微波热疗（δ）和X射线辐射（X）用作治疗剂。与未治疗的荷瘤对照小鼠骨髓细胞中每细胞的SCE值相比，同时接受这些三联疗法（MIS + δ + X）治疗的小鼠肿瘤的骨髓细胞中诱导出了显著（P小于0.05）数量的SCE。在同时接受MIS + δ + X治疗的小鼠肿瘤的骨髓细胞中，还观察到了最高数量的染色体畸变和改变，包括异倍体增加。对dbrB肿瘤的三联疗法还导致骨髓细胞中出现异常高的由320条染色体组成的多倍体中期板，表明这种治疗方式可能直接作用于荷瘤宿主细胞的遗传物质，作为副作用诱导细胞遗传学异常。
