Taylor L, Schneider E, Smith J, Polgar P
Mech Ageing Dev. 1981;16(4):311-7. doi: 10.1016/0047-6374(81)90015-4.
Prostaglandin (PG) production by human embryo lung fibroblasts (HELF) is stimulated by a number of effectors including angiotensin, thrombin, bradykinin and ascorbic acid. The types of prostaglandins produced are to a degree effector-dependent. For example, angiotensin stimulates mainly PGE2 synthesis, thrombin stimulates production of both PGE2 and prostacyclin while bradykinin and ascorbic acid stimulate production of PGE2, PGF2 alpha, prostacyclin and thromboxane A2. Upon senescence in culture, prostacyclin (PGI2) production by HELF drops dramatically in response to ascorbic or arachidonic acids. An overall drop in prostaglandin synthesis is observed with bradykinin stimulation. Prostaglandin production is also related to senescence in human skin fibroblasts (HSF). These cells produce predominantly PGI2. Prostacyclin production in response to bradykinin drops in HSF as they are obtained from individuals of increasing chronologic age. Thus our results indicate changes in prostaglandin production upon senescence, a dependency of these changes on the given stimulus and a correlation between in vivo and in culture aging with respect to prostaglandin production.
人胚肺成纤维细胞（HELF）产生前列腺素（PG）受到多种效应物的刺激，包括血管紧张素、凝血酶、缓激肽和抗坏血酸。所产生的前列腺素类型在一定程度上依赖于效应物。例如，血管紧张素主要刺激PGE2的合成，凝血酶刺激PGE2和前列环素的产生，而缓激肽和抗坏血酸刺激PGE2、PGF2α、前列环素和血栓素A2的产生。在培养过程中细胞衰老时，HELF对抗坏血酸或花生四烯酸的反应中前列环素（PGI2）的产生急剧下降。缓激肽刺激时可观察到前列腺素合成总体下降。前列腺素的产生也与人类皮肤成纤维细胞（HSF）的衰老有关。这些细胞主要产生PGI2。从年龄越来越大的个体获取的HSF中，对缓激肽反应产生的前列环素下降。因此，我们的结果表明衰老时前列腺素产生的变化、这些变化对特定刺激的依赖性以及体内和体外培养衰老在前列腺素产生方面的相关性。
