Yayanos A A, Dietz A S, Van Boxtel R
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5212-5. doi: 10.1073/pnas.78.8.5212.
An amphipod (Hirondellea gigas) was retrieved with decompression in an insulated trap from an ocean depth of 10,476 m. Bacterial isolates were obtained from the dead and cold animal by using silica gel medium incubated at 1000 bars (1 bar = 10(5) Pa) and 2 degrees C. The isolate designated MT41 was found to be obligately barophilic and did not grow at a pressure close to that of 380 bars found at average depths of the sea. The optimal generation time of about 25 hr was at 2 degrees C and 690 bars. The generation time at 2 degrees C and 1,035 bars, a pressure close to that at the depth of origin, was about 33 hr. Among the conclusions are: (i) pressure is an important determinant of zonation along the water column of the sea; (ii) some obligately barophilic bacteria survive decompressions; (iii) the pressure of optimal growth at 2 degrees C appears to be less than the pressure at the depth of origin and may be diagnostic for the depth of origin; (iv) rates of reproduction are slow yet significant and an order of magnitude greater than previously thought; and (v) much of deep-sea microbiology may have been done with spurious deep-sea organisms due to warming of samples.
在一个隔热诱捕器中，从10476米的海洋深处减压回收了一只等足类动物（巨尾深渊等足虫）。通过在1000巴（1巴 = 10⁵帕斯卡）和2摄氏度下培养的硅胶培养基，从这只死亡且冰冷的动物身上获得了细菌分离株。被命名为MT41的分离株被发现是专性嗜压菌，在接近海洋平均深度所发现的380巴的压力下无法生长。其最佳代时约为25小时，是在2摄氏度和690巴的条件下。在2摄氏度和1035巴（接近其起源深度的压力）下的代时约为33小时。研究得出的结论包括：（i）压力是海洋水柱中生物分布带的一个重要决定因素；（ii）一些专性嗜压菌能在减压过程中存活；（iii）在2摄氏度下最佳生长的压力似乎低于其起源深度的压力，这可能有助于判断其起源深度；（iv）繁殖速率虽慢但显著，比之前认为的快一个数量级；（v）由于样本升温，许多深海微生物学研究可能使用了虚假的深海生物。
