Yayanos A A, Pollard E C
Biophys J. 1969 Dec;9(12):1464-82. doi: 10.1016/S0006-3495(69)86466-0.
In cultures of Escherichia coli 15 (thymine(-), leucine(-)) which were incubated at high hydrostatic pressures, cell division occurred only at pressures below 430 atm but in a somewhat synchronous fashion at around 250 atm. The rate of leucine-(14)C incorporation into a macromolecular fraction of the cells diminished to a zero value at about 580 atm and that of uracil-(14)C incorporation to a zero value at about 770 atm. The rate of thymine-(14)C incorporation at pressures around 330 atm was that to be expected with a culture in which DNA synthesis is somewhat synchronous. At pressures above 500 atm, thymine-(14)C was incorporated only over the initial part of the pressure incubation and further incorporation under pressure was not observed no matter how long the duration of the incubation. We present evidence along several lines that the thymine incorporation kinetics reflect an effect of pressure on a locus at the origin (or termination) of a replication of the bacterial chromosome. The recovery of cell division and of the incorporation rates upon release of pressure were found to depend on the magnitude of the pressure and the duration of the pressure incubation.
在高压下培养的大肠杆菌15（胸腺嘧啶（-），亮氨酸（-））中，细胞分裂仅在低于430个大气压时发生，但在约250个大气压时以某种同步方式进行。细胞大分子部分中亮氨酸-（14）C的掺入率在约580个大气压时降至零值，尿嘧啶-（14）C的掺入率在约770个大气压时降至零值。在约330个大气压下，胸腺嘧啶-（14）C的掺入率是DNA合成有点同步的培养物所预期的。在高于500个大气压时，胸腺嘧啶-（14）C仅在压力培养的初始阶段掺入，无论培养持续多长时间，在压力下都未观察到进一步掺入。我们从几个方面提供证据表明，胸腺嘧啶掺入动力学反映了压力对细菌染色体复制起点（或终点）位点的影响。发现压力释放后细胞分裂和掺入率的恢复取决于压力大小和压力培养持续时间。
