流体静压力对大肠杆菌培养物中细胞分裂的刺激作用。

Stimulatory effect of hydrostatic pressure on cell division in cultures of Escherichia coli.

作者信息

Yayanos A A

出版信息

Biochim Biophys Acta. 1975 Jun 12;392(2):271-5. doi: 10.1016/0304-4165(75)90008-2.

Abstract

[14-C] Thymine incorporation into a trichloracetic acid-insoluble fraction of Escherichia coli HMS83 polA1 polB1 and cell division were observed as a function of time. The data presented show that hydrostatic pressures of about 600 x 10-5 N/m-2 stimulate that part of the division process which leads to the separation of cells. The kinetics of [14-C] thymine incorporation by this mutant suggest that completion of rounds of replication at high pressures is not dependent on DNA polymerases I or II or at least on high levels of these enzymes.

摘要

观察了[14-C]胸腺嘧啶掺入大肠杆菌HMS83 polA1 polB1的三氯乙酸不溶性部分以及细胞分裂随时间的变化情况。所呈现的数据表明，约600×10-5 N/m-2的静水压力刺激了导致细胞分离的那部分分裂过程。该突变体对[14-C]胸腺嘧啶的掺入动力学表明，在高压下完成复制轮次并不依赖于DNA聚合酶I或II，或者至少不依赖于这些酶的高水平表达。