Sartorelli A C
Biochim Biophys Acta. 1981 Nov 20;649(1):105-12. doi: 10.1016/0005-2736(81)90014-6.
Cell surface alterations occurred during murine erythroleukemia cell (clone 745) differentiation that were detected by both agglutination and lectin binding. Agglutination of erythroleukemia cells was produced by wheat germ agglutinin; whereas, concanavalin A, Ricin, soybean agglutinin and fucose-binding protein were either ineffective or much less efficacious. Treatment of leukemia cells with the inducer of erythroid differentiation dimethylsulfoxide (DMSO) caused a progressive accumulation of hemoglobin-containing cells in culture and a decrease in the rate of agglutination by wheat germ agglutinin, which began at 24 h after exposure to the polar solvent, reached a nadir at 48 h, and remained essentially constant thereafter. The binding of radioactive wheat germ agglutinin by untreated control erythroleukemia cells increased with time in culture, reaching a maximum value at 48 h, and decreased progressively thereafter. Although an increase in 3H-labeled wheat germ agglutinin binding also occurred in DMSO-treated cells, the level bound was significantly lower than that observed in control cells at 24-96 h. The treatment of erythroleukemia cells with various concentrations of DMSO resulted in a decrease in the number of wheat germ agglutinin receptor sites. Other inducers of differentiation (i.e., dimethylformamide, bis(acetyl)diaminopentane) also inhibited the rate of wheat germ agglutinin-induced agglutination of erythroleukemia cells while, in contrast, the inducer tetramethylurea did not. These studies indicate that membrane changes occur during differentiation and suggest that there may be more than one mechanism involved in the initiation of maturation which ultimately leads to the common pathway of erythroid development.
在小鼠红白血病细胞（克隆745）分化过程中发生了细胞表面改变，这可通过凝集和凝集素结合检测到。小麦胚凝集素可使红白血病细胞发生凝集；而伴刀豆球蛋白A、蓖麻毒素、大豆凝集素和岩藻糖结合蛋白则无效或效果差得多。用红系分化诱导剂二甲基亚砜（DMSO）处理白血病细胞，导致培养物中含血红蛋白的细胞逐渐积累，且小麦胚凝集素引起的凝集速率降低，这种降低在暴露于极性溶剂后24小时开始，48小时达到最低点，此后基本保持恒定。未处理的对照红白血病细胞对放射性小麦胚凝集素的结合随培养时间增加，在48小时达到最大值，此后逐渐降低。虽然在DMSO处理的细胞中也出现了3H标记的小麦胚凝集素结合增加，但在24 - 96小时时，其结合水平明显低于对照细胞。用不同浓度的DMSO处理红白血病细胞导致小麦胚凝集素受体位点数量减少。其他分化诱导剂（即二甲基甲酰胺、双（乙酰）二氨基戊烷）也抑制小麦胚凝集素诱导的红白血病细胞凝集速率，而相比之下，诱导剂四甲基脲则没有。这些研究表明，在分化过程中会发生膜变化，并提示在启动成熟过程中可能涉及多种机制，最终导致红系发育的共同途径。
