Tsiftsoglou A S, Sartorelli A C
Biochim Biophys Acta. 1981 Apr 27;653(2):226-35. doi: 10.1016/0005-2787(81)90158-1.
Murine erythroleukemia cells, grown in culture and induced to differentiate by dimethylsulfoxide (DMSO), were employed to explore the relationship between cellular proliferation and maturation. The expression of an erythroid phenotype, as measured by the accumulation of hemoglobin, occurred over a narrow range of concentrations of inducers; the maximum degree of differentiation was attained at a level of inducing agent which caused slight inhibition of cell replication. Stationary phase cells with diminished capacity for DNA synthesis failed to differentiate in the presence of DMSO; whereas, in contrast, exponentially growing murine leukemia cells undergoing extensive DNA biosynthesis readily attained a differentiated phenotype. The induced synthesis of hemoglobin in Friend cells exposed to DMSO was inhibited by 5-bromo-2'-deoxyuridine, arabinosylcytosine and hydroxyurea, when cells were simultaneously exposed to DMSO and a metabolic inhibitor. However, addition of either 5-bromo-2'-deoxyuridine, arabinosylcytosine or hydroxyurea to cultures pretreated with DMSO did not prevent the ultimate expression of the erythroid phenotype. These findings suggest that a process which is associated with cellular proliferation is required for the initiation of murine erythroleukemia cell maturation, but not for the ultimate accumulation of the erythroid marker hemoglobin in cells programmed to differentiate.
在培养中生长并通过二甲基亚砜（DMSO）诱导分化的小鼠红白血病细胞，被用于探究细胞增殖与成熟之间的关系。通过血红蛋白的积累来衡量，红细胞表型的表达在诱导剂浓度的狭窄范围内发生；在导致细胞复制略有抑制的诱导剂水平下达到最大分化程度。DNA合成能力减弱的静止期细胞在DMSO存在下无法分化；然而，相比之下，正在进行大量DNA生物合成的指数生长的小鼠白血病细胞很容易达到分化表型。当细胞同时暴露于DMSO和代谢抑制剂时，暴露于DMSO的Friend细胞中血红蛋白的诱导合成受到5-溴-2'-脱氧尿苷、阿糖胞苷和羟基脲的抑制。然而，向用DMSO预处理的培养物中添加5-溴-2'-脱氧尿苷、阿糖胞苷或羟基脲并不能阻止红细胞表型的最终表达。这些发现表明，与细胞增殖相关的过程是小鼠红白血病细胞成熟起始所必需的，但对于已编程分化的细胞中红细胞标志物血红蛋白的最终积累并非必需。
