Hutchison C A, Singel D J
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6883-7. doi: 10.1073/pnas.78.11.6883.
We have used the electron paramagnetic resonance of Cu2+ bound in a tetragonal single crystal of hen egg-white lysozyme to obtain the electron nuclear double resonance spectra of protons in the vicinity of the Cu2+ at the site designated as B by Teichberg et al. [Teichberg, V. I., Sharon, N., Moult, J., Smilansky, A. & Yonath, A. (1974) J. Mol. Biol. 87, 357-368]. The values of the hyperfine interaction parameters and the coordinates of eight protons are reported. The configuration of the H2O molecules coordinated to the Cu2+ and their relationships to the protein molecule structure are discussed.
我们利用结合在鸡蛋清溶菌酶四方单晶中的Cu2+的电子顺磁共振，获得了在Teichberg等人[Teichberg, V. I., Sharon, N., Moult, J., Smilansky, A. & Yonath, A. (1974) J. Mol. Biol. 87, 357 - 368]指定为B位点的Cu2+附近质子的电子核双共振光谱。报道了超精细相互作用参数的值和八个质子的坐标。讨论了与Cu2+配位的H2O分子的构型及其与蛋白质分子结构的关系。
