Congote L F, Bruno F, Solomon S
Can J Biochem. 1977 May;55(5):571-5. doi: 10.1139/o77-081.
alpha-Fetoprotein and the synthesis of heme associated with hemoglobin were measured simultaneously in short-term cultures of human fetal liver cells to correlate the relationship of alpha-fetoprotein to erythroid cell function. Both synthetic processes decreased exponentially during the first 5 days of culture. The use of media supplemented with different batches of fetal calf serum and porcine portal vein serum indicated that the optimal conditions for the production of alpha-fetoprotein were different from those required for the synthesis of heme associated with hemoglobin. Moreover, the alpha-fetoprotein-producing cells could be separated from erythroid cells after velocity sedimentation in Ficoll gradients. Although it is well known that erythropoiesis and alpha-fetoprotein production occur simultaneously during ontogenesis, alpha-fetoprotein itself (0.01-100 micron g/ml) did not stimulate heme synthesis in liver erythroid cells. Erythropoietin did not stimulate alpha-fetoprotein production. It is concluded that there is no cause-effect relationship between alpha-fetoprotein production and erythroid cell fuction in human fetal liver cells and that the two processes occur independently in different cell types.
在人胎儿肝细胞的短期培养中，同时测定了甲胎蛋白和与血红蛋白相关的血红素合成，以关联甲胎蛋白与红细胞系细胞功能的关系。在培养的头5天，这两个合成过程均呈指数下降。使用添加了不同批次胎牛血清和猪门静脉血清的培养基表明，产生甲胎蛋白的最佳条件与合成与血红蛋白相关的血红素所需的条件不同。此外，在Ficoll梯度中进行速度沉降后，产生甲胎蛋白的细胞可与红细胞系细胞分离。虽然众所周知，在个体发育过程中红细胞生成和甲胎蛋白产生同时发生，但甲胎蛋白本身（0.01 - 100微克/毫升）并未刺激肝脏红细胞系细胞中的血红素合成。促红细胞生成素也未刺激甲胎蛋白的产生。得出的结论是，人胎儿肝细胞中甲胎蛋白产生与红细胞系细胞功能之间不存在因果关系，这两个过程在不同细胞类型中独立发生。
