Megias M, Caviedes M A, Palomares A J, Perezsilva J
J Bacteriol. 1982 Jan;149(1):59-64. doi: 10.1128/jb.149.1.59-64.1982.
Plasmid R68.45 was used to promote conjugal transfer of chromosomal markers in Rhizobium trifolii RS55. Analysis of two-factor and three-factor crosses among R. trifolii strains enabled construction of a circular linkage map of the R. trifolii chromosome, containing 17 nutritional and resistance markers.
质粒R68.45被用于促进三叶草根瘤菌RS55中染色体标记的接合转移。对三叶草根瘤菌菌株间的双因子和三因子杂交进行分析,构建了一张三叶草根瘤菌染色体的环状连锁图谱,其中包含17个营养和抗性标记。