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J Bacteriol. 1980 Jan;141(1):121-8. doi: 10.1128/jb.141.1.121-128.1980.
2
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8
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10
The isolated N-terminal DNA binding domain of the c repressor of bacteriophage 16-3 is functional in DNA binding in vivo and in vitro.噬菌体16 - 3的c阻遏物的分离的N端DNA结合结构域在体内和体外的DNA结合中均具有功能。
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A proposal for a uniform nomenclature in bacterial genetics.细菌遗传学统一命名法的提议。
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Genetic mapping of rhizobiophage 16-3.根瘤菌噬菌体16-3的遗传图谱
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Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.在与一类大肠杆菌K12 Rec-受体菌株交配时形成部分二倍体。
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Genetic studies on rhizobiophage 16-3. I. Genes and functions on the chromosome.根瘤菌噬菌体16-3的遗传学研究。I. 染色体上的基因与功能
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Escherichia coli K-12 F-prime factors, old and new.大肠杆菌K-12 F-prime因子，新旧情况
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8
Evidence for common genetic determinants of nitrogenase and nitrate reductase in Rhizobium meliloti.苜蓿根瘤菌中固氮酶和硝酸还原酶共同遗传决定因素的证据。
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Genetic studies on rhizobiophage 16-3. II. Helper-induced transfection.根瘤菌噬菌体16 - 3的遗传学研究。II. 辅助诱导转染。
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Escherichia coli gene transfer to unrelated bacteria by a histidine operon--RP1 drug resistance plasmic complex.
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从苜蓿根瘤菌中分离并鉴定一个R-prime质粒。

Isolation and characterization of an R-prime plasmid from Rhizobium meliloti.

作者信息

Kiss G B, Dobo K, Dusha I, Breznovits A, Orosz L, Vincze E, Kondorosi A

出版信息

J Bacteriol. 1980 Jan;141(1):121-8. doi: 10.1128/jb.141.1.121-128.1980.

DOI:10.1128/jb.141.1.121-128.1980
PMID:6986354
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC293544/
Abstract

Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46+, and att16-3. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The att16-3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria.

摘要

通过一种简单的富集程序，我们分离出了质粒R68.45的一个R-prime衍生物，它携带了苜蓿根瘤菌41染色体的一个17.8兆道尔顿片段。此质粒（pGY1）上携带的染色体片段包括标记cys-24+、cys-46+和att16-3。质粒pGY1从同源区域开始以极化方式动员染色体，但不能促进从其他位点进行染色体转移。pGY1上的att16-3位点允许噬菌体16-3整合到pGY1中，从而形成了一个91.8兆道尔顿的复合质粒。该载体（pGY2）适用于将苜蓿根瘤菌噬菌体16-3导入其他革兰氏阴性细菌。