Hunt R C, Hood J A
Biochim Biophys Acta. 1982 Feb 10;720(1):106-10. doi: 10.1016/0167-4889(82)90044-1.
When human erythroleukemic cells are induced to differentiate in vitro, the lipids in the plasma membrane that bind the fluorescent dye merocyanine 540 are redistributed into a cap at one pole of the cell. This capping phenomenon can also be observed in uninduced cells that have been incubated with cytochalasin B, an agent which disrupts actin-containing microfilaments or with local anesthetics which act on both microfilaments and microtubules. Colchicine which acts on microtubules, however, has no effect. This suggests that the uniform distribution seen in uninduced cells is maintained by the cytoskeletal microfilaments and that loss of these structures leads to spontaneous redistribution of merocyanine 540-binding sites.
当人红白血病细胞在体外被诱导分化时，质膜中与荧光染料部花青540结合的脂质会重新分布到细胞一极的帽状结构中。这种帽化现象在未诱导的细胞中也能观察到，这些未诱导的细胞已与细胞松弛素B（一种破坏含肌动蛋白的微丝的试剂）或与作用于微丝和微管的局部麻醉剂一起孵育。然而，作用于微管的秋水仙碱没有效果。这表明未诱导细胞中看到的均匀分布是由细胞骨架微丝维持的，并且这些结构的丧失导致部花青540结合位点的自发重新分布。
