Aiuchi T, Kobatake Y
J Membr Biol. 1979 Apr 9;45(3-4):233-44. doi: 10.1007/BF01869287.
The fluorescence of merocyanine 540 (MC) in liposomal and mitochondrial suspensions was measured under various conditions. Under a given condition, both the amount of dye bound to the membrane and the zeta potential were determined simultaneously. It was found that the fluorescence intensity was proportional to the amount of bound dye and correlated with the zeta potential of particles. The fluorescence intensity was represented quantiatively in terms of the Langmuir adsorption isotherm, when the electrostatic interaction acting between MC and membrane surface was properly taken into account. It was concluded that the changes in MC fluorescence in the liposomal and mitochondrial suspensions are mainly attributed to the changes in the surface potential of the membranes.
在各种条件下测量了脂质体和线粒体悬浮液中部花青540（MC）的荧光。在给定条件下，同时测定了与膜结合的染料量和ζ电位。发现荧光强度与结合染料的量成正比，并且与颗粒的ζ电位相关。当适当考虑MC与膜表面之间的静电相互作用时，荧光强度根据朗缪尔吸附等温线进行定量表示。得出的结论是，脂质体和线粒体悬浮液中MC荧光的变化主要归因于膜表面电位的变化。
