Schlegel R A, Phelps B M, Waggoner A, Terada L, Williamson P
Cell. 1980 Jun;20(2):321-8. doi: 10.1016/0092-8674(80)90618-2.
Normal erythroid cells and both uninduced and induced erythroleukemia cells were stained with the leukemia-specific fluorescent probe merocyanine 540 and its analogs. The external membranes of normal intact cells bound the dye, but this general low-affinity binding was completely abolished by the addition of competing serum. In contrast, erythroleukemia cells bound the dye even in the presence of serum; binding was not affected by reversing the sign of the charge carried by MC540, but was abolished upon removal of certain hydrophobic side chains. When the erythroleukemia cells were induced to differentiate, the distribution of dye-binding regions was altered by the cell such that staining became localized to one region of the membrane. Concomitantly, conconavalin A binding sites were redistributed and became localized in the same region of the membrane as the merocyanine binding sites. Merocyanine 540 is thus shown to bind to a hematopoietic surface feature whose topological distribution is subject to cellular control during differentiation. This leukemia-specific marker may be one of several eliminated during enucleation of mammalian erythroid cells.
正常红细胞以及未诱导和诱导的红白血病细胞都用白血病特异性荧光探针部花青540及其类似物进行染色。正常完整细胞的外膜能结合该染料，但加入竞争性血清后，这种普遍的低亲和力结合完全被消除。相比之下，即使存在血清，红白血病细胞仍能结合染料；MC540所带电荷符号反转时，结合不受影响，但去除某些疏水侧链后结合被消除。当红白血病细胞被诱导分化时，细胞会改变染料结合区域的分布，使染色定位于膜的一个区域。同时，伴刀豆球蛋白A结合位点重新分布，并定位于与部花青结合位点相同的膜区域。因此表明，部花青540能结合造血表面特征，其拓扑分布在分化过程中受细胞控制。这种白血病特异性标志物可能是哺乳动物红细胞去核过程中被消除的几种标志物之一。
