Simon G, Conklin D J, Soong L B
Clin Exp Hypertens (1978). 1981;3(6):1183-94. doi: 10.3109/10641968109037432.
The serum of perinephritic hypertensive dogs causes waterlogging and increased sodium (Na) content of rabbit aorta explants in tissue culture. In the present study, we investigated the role of tissue glycosaminoglycans in the pathogenesis of these changes. The effect of ouabain and prostaglandin F2 alpha on the composition of rabbit aorta explants in tissue culture was studied to determine if they produced alterations that were similar to those produced by the serum of hypertensive dogs. Rabbit aortic media explants were cultured in tissue culture medium supplemented (15-20%) with serum obtained from 12 dogs during a pre-hypertension control period and after induction of one-kidney, one-wrapped hypertension. After 3 weeks of culture, the explants were harvested, and their Na, potassium (K) and hexosamine content was measured. Compared to the composition of explants cultured in pre-hypertension control serum, the water and Na content of explants cultured in the serum of hypertensive dogs was increased (p less than 0.02, and p less than 0.01). There were no differences in the K and hexosamine content of explants cultured in the two sera. There was a dose-dependent increase in the NA content and a reduction in the K content of explants cultured in the presence of ouabain (5 X 10-8 to 5 X 10-7 M) and PGF2 alpha (1 microgram/ml). The findings of this study provide further evidence for angiopathic serum factor(s) in perinephritic hypertensive dogs. The excess Na of explants cultured in the serum of hypertensive dogs does not appear to be bound to glycosaminoglycans, The accumulation of excess Na is not due to an ouabain-like mechanism.
肾盂肾炎性高血压犬的血清可导致兔主动脉外植体在组织培养中出现水潴留和钠（Na）含量增加。在本研究中，我们调查了组织糖胺聚糖在这些变化发病机制中的作用。研究了哇巴因和前列腺素F2α对兔主动脉外植体在组织培养中成分的影响，以确定它们是否产生与高血压犬血清所产生的类似改变。兔主动脉中膜外植体在补充（15 - 20%）有来自12只犬在高血压前期对照期以及诱导一侧肾、一侧包裹性高血压后获得的血清的组织培养基中培养。培养3周后，收获外植体，并测量其钠、钾（K）和己糖胺含量。与在高血压前期对照血清中培养的外植体成分相比，在高血压犬血清中培养的外植体的水和钠含量增加（p < 0.02，p < 0.01）。在两种血清中培养的外植体的钾和己糖胺含量没有差异。在存在哇巴因（5×10 - 8至5×10 - 7 M）和前列腺素F2α（1微克/毫升）的情况下培养的外植体，其钠含量呈剂量依赖性增加，钾含量降低。本研究结果为肾盂肾炎性高血压犬存在血管病变血清因子提供了进一步证据。在高血压犬血清中培养的外植体中过量的钠似乎未与糖胺聚糖结合。过量钠的积累不是由于类似哇巴因的机制。
