Simon G, Conklin D J, Altman S
J Lab Clin Med. 1986 May;107(5):439-46.
The long-term cellular metabolic effects of serum factors from renal hypertensive and uninephrectomized normotensive dogs were investigated. Mouse fibroblastic L cells were cultured in M199 tissue culture medium supplemented (to 15%) with serum obtained from dogs before and after the induction of one-kidney one wrapped hypertension or with serum obtained from normotensive dogs before and after unilateral nephrectomy. At confluency, the protein, water (3-O-methyl-14C-D-glucose space), Na, K, and Mg content and 3H-thymidine uptake of cells were measured. Growth curves were derived for cells growing in serum obtained before and after uninephrectomy from normotensive and hypertensive dogs. Postnephrectomy serum from both normotensive and hypertensive dogs resulted in increased recovery of protein, increased water, K, and Mg content of cells, and increased cell numbers. 3H-thymidine uptake at confluency in postnephrectomy serum was the same as in prenephrectomy serum. The one important qualitative difference between postnephrectomy serum from normotensive and hypertensive dogs was the reduced Na content and concentration of cells cultured in the serum of hypertensive dogs. The growth-promoting serum factor in postnephrectomy blood was detected for up to 8 weeks after uninephrectomy; therefore, it did not appear to be renotropin. In experimental renal hypertension, there appear to be previously unidentified serum factors that enhance transmembrane Na gradient.
研究了肾性高血压犬和单侧肾切除的正常血压犬血清因子的长期细胞代谢效应。将小鼠成纤维细胞L在添加（至15%）了从诱导一侧肾包裹性高血压前后的犬获得的血清或从单侧肾切除前后的正常血压犬获得的血清的M199组织培养基中培养。在汇合时，测量细胞的蛋白质、水（3-O-甲基-14C-D-葡萄糖空间）、钠、钾和镁含量以及3H-胸腺嘧啶核苷摄取量。得出了在正常血压和高血压犬单侧肾切除前后获得的血清中生长的细胞的生长曲线。正常血压和高血压犬肾切除后的血清均导致蛋白质回收率增加、细胞水、钾和镁含量增加以及细胞数量增加。肾切除后血清中汇合时的3H-胸腺嘧啶核苷摄取量与肾切除前血清中的相同。正常血压和高血压犬肾切除后血清之间一个重要的定性差异是，在高血压犬血清中培养的细胞钠含量和浓度降低。肾切除后血液中的生长促进血清因子在单侧肾切除后长达8周均可检测到；因此，它似乎不是促肾生长素。在实验性肾性高血压中，似乎存在以前未鉴定的血清因子，可增强跨膜钠梯度。
