Simon G
Hypertension. 1979 May-Jun;1(3):197-201. doi: 10.1161/01.hyp.1.3.197.
To investigate the role of circulating humoral substances in the pathogenesis of increased vascular wall water and sodium concentration in experimental hypertension, rabbit aortic media explants were cultured in tissue culture medium supplemented (10-20%) with serum obtained from the same dogs (n = 7): 1) before the induction of hypertension; 2) after wrapping one kidney in silk (two-kidney perinephritic hypertension, 2-KPHT); and 3) after contralateral nephrectomy (1-KPHT). Cultures also were run with serum of sham-wrapped and then unilaterally nephrectomized normotensive dogs (n = 4). After 3 weeks of culture, the explants were harvested, and their water, sodium and potassium concentration was measured. Compared to the composition of explants cultured in prehypertensive serum, the water concentration of explants cultured in 1-KPHT and the sodium concentration of explants cultured in 2-KPHT and 1-KPHT serum were increased (p < 0.05). The water and electrolyte content of explants cultured in sera of sham-operated normotensive control dogs was the same regardless of the type of serum used, pre- or post-sham surgery or post-nephrectomy. The effects of serum from hypertensive dogs were not explained by variations in serum creatinine, sodium and potassium levels or in plasma renin activities. The experiments provide evidence for the role of serum factor(s) in the pathogenesis of abnormal vascular wall water and sodium concentration in experimental hypertension.
为了研究循环体液物质在实验性高血压中血管壁水钠浓度升高发病机制中的作用，将兔主动脉中膜外植体培养于添加了（10 - 20%）取自相同犬只（n = 7）血清的组织培养基中：1）高血压诱导前；2）一侧肾脏用丝线包裹后（双肾周围性高血压，2 - KPHT）；3）对侧肾切除后（1 - KPHT）。还使用假包裹然后单侧肾切除的正常血压犬只（n = 4）的血清进行培养。培养3周后，收获外植体并测量其水、钠和钾浓度。与在高血压前血清中培养的外植体成分相比，在1 - KPHT血清中培养的外植体的水浓度以及在2 - KPHT和1 - KPHT血清中培养的外植体的钠浓度均升高（p < 0.05）。无论使用何种血清类型，假手术正常血压对照犬血清培养的外植体的水和电解质含量相同，无论是假手术前或后还是肾切除后。高血压犬血清的作用不能用血清肌酐、钠和钾水平或血浆肾素活性的变化来解释。这些实验为血清因子在实验性高血压中血管壁水钠浓度异常发病机制中的作用提供了证据。
