Okano H, Okamura J, Yagawa K, Tasaka H, Motomura S
J Cancer Res Clin Oncol. 1981;102(1):49-55. doi: 10.1007/BF00410534.
Three continuous human cell lines, designated KMOE, derived from a patient with acute erythremia (Di Guglielmo's disease) are reported. The cell lines are the cultures of (1) bone marrow cells, (2) peripheral blood cells, and (3) cells from a tumor developed into an athymic nude mouse after transplantation of the cultured bone marrow cells. Cells of all three lines show morphology of immature erythroblast and have i(17q) marker chromosome. They are negative for both Philadelphia chromosome and Epstein-Barr virus nuclear antigen. Although all KMOE cells in suspension culture are benzidine-negative, benzidine-positive cells are found within colonies formed in semi-solid culture media. The relative number of colonies with benzidine-positive cells is increased when sodium butyrate is added to the culture.
报道了源自一名急性红细胞增多症(迪古列尔莫氏病)患者的三种连续人类细胞系,命名为KMOE。这些细胞系分别是:(1)骨髓细胞培养物,(2)外周血细胞培养物,以及(3)将培养的骨髓细胞移植到无胸腺裸鼠后形成的肿瘤细胞培养物。所有三个细胞系的细胞均显示未成熟成红细胞的形态,并具有i(17q)标记染色体。它们对费城染色体和爱泼斯坦-巴尔病毒核抗原均呈阴性。尽管悬浮培养中的所有KMOE细胞对联苯胺呈阴性,但在半固体培养基中形成的集落内发现了联苯胺阳性细胞。当向培养物中添加丁酸钠时,联苯胺阳性细胞的集落相对数量增加。