嗜热四膜虫中DNA腺嘌呤甲基化酶的序列特异性
Sequence specificity of DNA adenine methylase in the protozoan Tetrahymena thermophila.
作者信息
Bromberg S, Pratt K, Hattman S
出版信息
J Bacteriol. 1982 May;150(2):993-6. doi: 10.1128/jb.150.2.993-996.1982.
Abstract
The sequence specificity of the Tetrahymena DNA-adenine methylase was determined by nearest-neighbor analyses of in vivo and in vitro methylated DNA. In vivo all four common bases were found to the 5' side of N6-methyladenine, but only thymidine was 3'. Homologous DNA already methylated in vivo and heterologous Micrococcus luteus DNA were methylated in vitro by a partially purified DNA-adenine methylase activity isolated from Tetrahymena macronuclei. The in vitro-methylated sequence differed from the in vivo sequence in that both thymidine and cytosine were 3' nearest neighbors of N6-methyladenine.
摘要
通过对体内和体外甲基化DNA的邻位分析，确定了嗜热四膜虫DNA腺嘌呤甲基转移酶的序列特异性。在体内，发现所有四种常见碱基都在N6 - 甲基腺嘌呤的5' 侧，但只有胸腺嘧啶在3' 侧。体内已甲基化的同源DNA和异源藤黄微球菌DNA在体外被从嗜热四膜虫大核中分离出的部分纯化的DNA腺嘌呤甲基转移酶活性甲基化。体外甲基化序列与体内序列的不同之处在于，胸腺嘧啶和胞嘧啶都是N6 - 甲基腺嘌呤的3' 邻位碱基。
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