真核生物梨形四膜虫核DNA中的（6 - N）甲基腺嘌呤。
( 6 N)methyl adenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis.
作者信息
Gorovsky M A, Hattman S, Pleger G L
出版信息
J Cell Biol. 1973 Mar;56(3):697-701. doi: 10.1083/jcb.56.3.697.
Abstract
DNA isolated from macronuclei of the ciliate, Tetrahymena pyriformis, has been found to contain [(6)N]methyl adenine (MeAde); this represents the first clear demonstration of significant amounts of MeAde in the DNA of a eucaryote. The amounts of macronuclear MeAde differed slightly between different strains of Tetrahymena, with approximately 0.65-0.80% of the adenine bases being methylated. The MeAde content of macronuclear DNA did not seem to vary in different physiological states. The level of MeAde in DNA isolated from micronuclei, on the other hand, was quite low (at least tenfold lower than in macronuclear DNA).
摘要
从梨形四膜虫这种纤毛虫的大核中分离出的DNA已被发现含有[(6)N]甲基腺嘌呤（MeAde）；这是首次明确证明真核生物的DNA中存在大量MeAde。不同品系的四膜虫大核中的MeAde含量略有差异，约0.65 - 0.80%的腺嘌呤碱基被甲基化。大核DNA的MeAde含量在不同生理状态下似乎没有变化。另一方面，从微核中分离出的DNA中的MeAde水平相当低（至少比大核DNA中的低十倍）。
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本文引用的文献
1
Recognition and estimation of 5-methylcytosine in nucleic acids.核酸中5-甲基胞嘧啶的识别与估算。
Biochem J. 1951 May;48(5):581-4. doi: 10.1042/bj0480581.
2
Methylation of nucleic acids in Krebs II ascites tumour cells.克雷布斯II腹水瘤细胞中核酸的甲基化作用
Nature. 1966 May 21;210(5038):797-9. doi: 10.1038/210797a0.
3
The fine structure of the nuclei of Tetrahymena pyriformis throughout the cell cycle.梨形四膜虫细胞核在整个细胞周期中的精细结构。
J Cell Biol. 1965 Dec;27(3):519-29. doi: 10.1083/jcb.27.3.519.
4
Deoxyribonucleic acid methylase activity in pea seedlings.豌豆幼苗中的脱氧核糖核酸甲基酶活性。
Science. 1969 May 9;164(3880):721-2. doi: 10.1126/science.164.3880.721.
5
The in vivo methylation of DNA in mouse fibroblasts.小鼠成纤维细胞中DNA的体内甲基化。
Biochim Biophys Acta. 1969 Jan 21;174(1):322-9. doi: 10.1016/0005-2787(69)90257-3.
6
Methylation of DNA in developing sea urchin embryos.发育中的海胆胚胎中DNA的甲基化
J Mol Biol. 1968 Sep 14;36(2):195-208. doi: 10.1016/0022-2836(68)90375-6.
7
Methylation of Lilium DNA during the meiotic cycle.百合DNA在减数分裂周期中的甲基化
Biochim Biophys Acta. 1971 Apr 29;238(1):50-9. doi: 10.1016/0005-2787(71)90008-6.
8
Methylation of the deoxyribonucleic acid of Physarum polycephalum at various periods during the mitotic cycle.多头绒泡菌有丝分裂周期不同阶段脱氧核糖核酸的甲基化
J Biol Chem. 1970 Dec 10;245(23):6436-41.
9
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J Cell Biol. 1970 Dec;47(3):619-30. doi: 10.1083/jcb.47.3.619.
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