Bovbjerg D, Ader R, Cohen N
Proc Natl Acad Sci U S A. 1982 Jan;79(2):583-5. doi: 10.1073/pnas.79.2.583.
Cyclophosphamide (CY), previously used to condition suppression of humoral immune responses, was used to condition suppression of a graft-versus-host response (GvHR). Female (Lewis x Brown Norway) F1 rats were conditioned by pairing consumption of a saccharin solution with an intraperitoneal injection of CY at 50 mg/kg of body weight 48 days before immunization. On day 0, all animals were injected with a suspension of splenic leukocytes (2 x 10(7) cells per footpad) obtained from female Lewis donors. The regional GvHR was assessed on day 5 by weighing popliteal nodes. Conditioned animals given a single low-dose injection of CY and reexposed to conditioned stimuli had lymph node weights significantly lower than control groups and did not differ from animals given three injections of CY during the ongoing GvHR. The results suggest that conditioned immunosuppression, previously demonstrated in thymus-dependent and thymus-independent humoral immune responses, also affects the popliteal GvHR, a cellular immune response.
环磷酰胺（CY），此前用于调节对体液免疫反应的抑制，现被用于调节对移植物抗宿主反应（GvHR）的抑制。雌性（Lewis×Brown Norway）F1大鼠在免疫前48天，通过将饮用糖精溶液与腹腔注射50mg/kg体重的CY配对来进行预处理。在第0天，所有动物均注射从雌性Lewis供体获得的脾细胞悬液（每足垫2×10⁷个细胞）。在第5天通过称量腘窝淋巴结来评估局部GvHR。接受单次低剂量CY注射并再次暴露于条件刺激的预处理动物，其淋巴结重量显著低于对照组，且与在进行性GvHR期间接受三次CY注射的动物无差异。结果表明，先前在依赖胸腺和不依赖胸腺的体液免疫反应中证实的条件性免疫抑制，也影响腘窝GvHR，一种细胞免疫反应。
