Duff D A, Snell K
J Nutr. 1982 Jun;112(6):1057-66. doi: 10.1093/jn/112.6.1057.
Rats were overnourished during suckling by litter-size manipulation in order to investigate the possible association of overfeeding in infancy with the development of obesity in later life. Rats were raised in litters of 4, 10 and 16 corresponding to overfeeding, normal feeding and underfeeding during the suckling period. From 6-19 days post partum, growth rates of pups from different litter sizes were significantly different (4 greater than 10 greater than 16). Differences in mean body weights between the groups continued to increase after weaning when all groups were allowed access to diet ad libitum and the significant weight difference between overfed and normally fed rats persisted into adult life in both males and females. Overfed animals showed modifications in the development of activities of a number of hepatic enzymes involved in lipid and carbohydrate metabolism. In later life (20 weeks) neonatally overnourished rats exhibited alterations in hepatic enzyme activities that reflected an increased capacity for lipid synthesis by the liver. "Supernourishment" of neonatal rats (by intubation with glucose of animals in small litters), accelerated the appearance of some of these alterations. These studies show that the pattern of early infant nutrition can profoundly influence the activities of liver enzymes in later, adult life.
为了研究婴儿期过度喂养与后期肥胖发展之间可能存在的关联，通过调整窝仔数对哺乳期大鼠进行过度营养喂养。将大鼠饲养在窝仔数分别为4只、10只和16只的环境中，分别对应哺乳期的过度喂养、正常喂养和喂养不足。产后6至19天，不同窝仔数的幼崽生长速度存在显著差异（4只组大于10只组大于16只组）。断奶后，当所有组都可以自由进食时，各组之间的平均体重差异继续增大，过度喂养和正常喂养的大鼠之间的显著体重差异在雄性和雌性大鼠成年后依然存在。过度喂养的动物在参与脂质和碳水化合物代谢的多种肝酶活性发展方面出现了改变。在后期生活（20周时），新生期过度营养喂养的大鼠肝酶活性发生改变，这反映出肝脏脂质合成能力增强。新生大鼠的“超营养”（通过给小窝仔数动物插管喂食葡萄糖）加速了其中一些改变的出现。这些研究表明，早期婴儿营养模式可对后期成年生活中的肝脏酶活性产生深远影响。
