Edgar D H, Creighton A M
J Natl Cancer Inst. 1982 Jun;68(6):977-80.
Two independently derived sublines of Syrian hamster BHK 21 S cells that demonstrate resistance to the antitumor agent ICRF 159 (NSC-129943) were fused to sensitive cells in both intraspecific and interspecific crosses, and the responses of the resultant hybrids to the drug were determined. In intraspecific hybrids from crosses involving either of the sublines, resistance to ICRF 159 was expressed codominantly ith the hybrid response being halfway between the parental responses. In interspecific hybrids from crosses with sensitive mouse cells, resistance was also expressed but to a lesser extent than in the intraspecific hybrids. However, subsequent passaging of an interspecific hybrid resulted in an increase in the degree of resistance, which appeared to be accompanied by a loss of telocentric mouse chromosomes. The results suggested that resistance in these lines was due to an alteration in the cellular target involved in the response to ICRF 159.
叙利亚仓鼠BHK 21 S细胞的两个独立衍生的亚系对抗肿瘤药物ICRF 159（NSC - 129943）具有抗性，在种内和种间杂交中与敏感细胞融合，并测定所得杂种对该药物的反应。在涉及任一亚系的杂交产生的种内杂种中，对ICRF 159的抗性呈共显性表达，杂种反应介于亲本反应之间。在与敏感小鼠细胞杂交产生的种间杂种中，抗性也有表达，但程度低于种内杂种。然而，种间杂种随后传代导致抗性程度增加，这似乎伴随着近端着丝粒小鼠染色体的丢失。结果表明，这些品系中的抗性是由于对ICRF 159反应中细胞靶点的改变所致。
