Djøseland O, de Besche A, Høglo S, Rennie P S
J Steroid Biochem. 1982 Mar;16(3):397-402. doi: 10.1016/0022-4731(82)90051-6.
The metabolism of steroid hormones in normal and neoplastic tissue has been studied in the human parotid gland. In vitro incubation of homogenate preparations with either testosterone, oestradiol or DHT resulted in the formation of the corresponding 17-keto metabolite as the major product. Little or no metabolism could be seen when androstenedione, oestrone or 5 alpha-androstan-3,17-dione were used as substrates, demonstrating that the 17 beta-hydroxysteroid dehydrogenase (17 beta-HSDH) in this tissue favours an oxidative reaction pathway, 17 beta-HSDH activity in normal and tumour tissue was compared using both testosterone and oestradiol as substrates. The activity varied between individuals but was, in all cases, significantly elevated in tumour tissue when this was compared with normal. In conclusion, (1) the human parotid gland contains steroid metabolizing enzymes which favour an oxidative reaction pathway, and (2) the 17 beta-HSDH activity is significantly elevated in neoplastic tissue.
已在人腮腺中研究了正常组织和肿瘤组织中甾体激素的代谢。用睾酮、雌二醇或双氢睾酮对匀浆制剂进行体外孵育，结果形成相应的17-酮代谢产物作为主要产物。当使用雄烯二酮、雌酮或5α-雄烷-3,17-二酮作为底物时，几乎看不到或根本没有代谢，这表明该组织中的17β-羟基甾体脱氢酶（17β-HSDH）倾向于氧化反应途径。以睾酮和雌二醇作为底物，比较了正常组织和肿瘤组织中的17β-HSDH活性。活性在个体之间有所不同，但在所有情况下，与正常组织相比，肿瘤组织中的活性均显著升高。总之，（1）人腮腺含有倾向于氧化反应途径的甾体代谢酶，（2）肿瘤组织中的17β-HSDH活性显著升高。
