Robison L L, Arthur D C, Ball D W, Danzl T J, Nesbit M E
Cancer Res. 1982 Oct;42(10):4289-92.
Ten long-term survivors of childhood acute lymphoblastic leukemia were studied to determine if cytogenetic abnormalities were present in lymphocytes following discontinuation of therapy. The study included patients diagnosed between 1969 and 1974 who had received radiation therapy and a minimum of 3 years systemic chemotherapy. At study, the patients had been off all therapy from 1.3 to 6.5 years (median, 4 years). Peripheral blood lymphocytes were examined for spontaneous chromosome breakage and sister chromatid exchanges. In addition, G-banded metaphase and prometaphase chromosomes were analyzed. Chromosome breakage was found to be within normal limits for all patients. Likewise, there was no significant difference between patients and controls with respect to sister chromatid exchange frequency. However, seven of the ten patients were found to have one or more cells with nonclonal karyotypic abnormalities. Our results indicate that although long-term survivors of childhood acute lymphoblastic leukemia treated with intensive radiation and combination chemotherapy do not demonstrate chromosome instability or DNA damage as measured by breakage and sister chromatid exchange, a majority of these patients have a subpopulation of lymphocytes with nonclonal chromosome abnormalities years after stopping therapy.
对10名儿童急性淋巴细胞白血病的长期幸存者进行了研究，以确定停止治疗后淋巴细胞中是否存在细胞遗传学异常。该研究纳入了1969年至1974年间被诊断出患有该病且接受过放射治疗和至少3年全身化疗的患者。在研究时，患者已停止所有治疗1.3至6.5年（中位数为4年）。对外周血淋巴细胞进行了自发染色体断裂和姐妹染色单体交换检测。此外，还分析了G带中期和早中期染色体。结果发现所有患者的染色体断裂均在正常范围内。同样，患者与对照组在姐妹染色单体交换频率方面也没有显著差异。然而，10名患者中有7名被发现有一个或多个细胞存在非克隆性核型异常。我们的结果表明，尽管接受强化放疗和联合化疗的儿童急性淋巴细胞白血病长期幸存者未表现出通过断裂和姐妹染色单体交换所测量的染色体不稳定性或DNA损伤，但这些患者中的大多数在停止治疗数年之后，其淋巴细胞亚群存在非克隆性染色体异常。
