Lundberg J M, Tatemoto K, Terenius L, Hellström P M, Mutt V, Hökfelt T, Hamberger B
Proc Natl Acad Sci U S A. 1982 Jul;79(14):4471-5. doi: 10.1073/pnas.79.14.4471.
In immunohistochemical studies using antisera to peptide YY (PYY), a 36 amino acid polypeptide isolated from porcine duodenum, it was found that PYY-like immunoreactivity occurred mainly in endocrine cells of the gastrointestinal mucosa. PYY-immunoreactive cells were particularly abundant in the distal intestine and have been observed in five species, including man. By radioimmunoassay it was found that, in the rat, the amount of PYY immunoreactivity was about 100-fold higher in the colon than in the duodenum. The chromatographic profiles of PYY immunoreactivity from the rat colon and porcine PYY on a SP-Sephadex ion exchanger were similar. Furthermore, serial dilutions of extracts from the rat colon and porcine PYY had parallel displacement curves in radioimmunoassay. Close intraarterial administration of PYY in cats caused an intestinal vasoconstriction and an inhibition of jejunal and colonic motility. Simultaneously there was a rise in systemic arterial blood pressure. These effects of PYY were also observed after pretreatment with adrenergic blocking agents. It is concluded that PYY is a gastrointestinal peptide that is present mainly in endocrine cells of distal intestine and that has effect on both intestinal motility and the cardiovascular system.
在用抗肽YY（PYY）抗血清进行的免疫组织化学研究中，PYY是一种从猪十二指肠分离出的36个氨基酸的多肽，发现PYY样免疫反应主要发生在胃肠粘膜的内分泌细胞中。PYY免疫反应细胞在远端肠道特别丰富，并且在包括人类在内的五个物种中都观察到了。通过放射免疫测定发现，在大鼠中，结肠中PYY免疫反应的量比十二指肠中高约100倍。大鼠结肠中PYY免疫反应的色谱图谱与猪PYY在SP-葡聚糖离子交换剂上的图谱相似。此外，大鼠结肠提取物和猪PYY的系列稀释液在放射免疫测定中有平行的置换曲线。在猫体内动脉内近距离注射PYY会引起肠道血管收缩以及空肠和结肠运动的抑制。同时，全身动脉血压会升高。在用肾上腺素能阻断剂预处理后也观察到了PYY的这些作用。得出的结论是，PYY是一种胃肠肽，主要存在于远端肠道的内分泌细胞中，并且对肠道运动和心血管系统都有影响。
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