一种针对人促红细胞生成素的单克隆抗体的特性鉴定

Weiss T L, Kavinsky C J, Goldwasser E

Proc Natl Acad Sci U S A. 1982 Sep;79(18):5465-9. doi: 10.1073/pnas.79.18.5465.

Hybrid cells that synthesize a monospecific antibody directed toward erythropoietin have been produced by the fusion of mouse plasmacytoma cells with spleen cells from rats immunized against human erythropoietin. The antibody binds the alpha and beta forms and the asialo alpha form of erythropoietin to the same extent. It is an immunoglobulin of the IgG class and binds only erythropoietin in an impure preparation of the hormone. Biologically active unlabeled erythropoietin competes with biologically inactive radiolabeled hormone for monoclonal antibody binding sites. In addition, the biological activity of erythropoietin measured in vitro is not inactivated when it is bound by the monoclonal antibody.

通过将小鼠浆细胞瘤细胞与经抗人促红细胞生成素免疫的大鼠脾细胞融合，已产生了合成针对促红细胞生成素的单特异性抗体的杂交细胞。该抗体与促红细胞生成素的α和β形式以及去唾液酸α形式的结合程度相同。它是一种IgG类免疫球蛋白，在激素的不纯制剂中仅与促红细胞生成素结合。具有生物活性的未标记促红细胞生成素与无生物活性的放射性标记激素竞争单克隆抗体结合位点。此外，促红细胞生成素在体外测得的生物活性在与单克隆抗体结合时不会失活。