Malchow-Møller A, Arffmann S, Larusso N F, Krag E
Scand J Gastroenterol. 1982 Apr;17(3):331-3. doi: 10.3109/00365528209182063.
A method for determining faecal bile acids, suitable for clinical purposes, is introduced. The analysis uses a 0.2-g stool specimen, a simple extraction procedure, and 3 alpha-steroid dehydrogenase determination. The method, which is rapid, has been validated by gas-liquid chromatography and by recovery of internal standards. Stool examination was done in 16 healthy volunteers on free diet and in 25 patients with non-gastrointestinal diseases who were on a fat- and fibre-fixed diet. No difference was found between the two groups, so the data were pooled, and the normal reference interval (mean +/- S.D.) for faecal bile acid output was calculated to be 0-975 mumol/24h.
介绍了一种适用于临床目的的粪便胆汁酸测定方法。该分析采用0.2克粪便标本、简单的提取程序和3α-类固醇脱氢酶测定法。该方法快速，已通过气液色谱法和内标回收率验证。对16名自由饮食的健康志愿者和25名患有非胃肠道疾病且采用固定脂肪和纤维饮食的患者进行了粪便检查。两组之间未发现差异，因此将数据合并，并计算出粪便胆汁酸排出量的正常参考区间（均值±标准差）为0 - 975微摩尔/24小时。
