Polak L
Ann N Y Acad Sci. 1982;392:90-106. doi: 10.1111/j.1749-6632.1982.tb36100.x.
Dinitrophenylated macrophages are efficient inducers of contact sensitivity and of tolerance to dinitrochlorobenzene, depending on the mode of application. Contact sensitivity induced by an intradermal injection of DNP-M phi is genetically restricted, whereas tolerance induced by an intravenous injection is, in guinea pigs, not under control. This tolerance is complete in the majority of animals, but is reversed by DNCB in Freund's complete adjuvant and prevented by pretreatment with cyclophosphamide. Tolerance induced by an intravenous injection of the unconjugated hapten is not reversed by DNCB in FCA, but is abrogated by an intradermal injection of DNP-M phi. This abrogation does not occur, however, when DNCB in FCA is applied simultaneously with the DNP-M phi. In further experiments, it is demonstrated that the genetic restriction of induction of contact sensitivity by DNP-M phi is on the level of antigen recognition and is not due to the nonidentity of the Ia structures on macrohages used for induction and the Ia structures involved in elicitation of contact sensitivity.
二硝基苯基化巨噬细胞是接触敏感性和对二硝基氯苯耐受性的有效诱导剂，这取决于应用方式。皮内注射二硝基苯基化巨噬细胞（DNP-M phi）诱导的接触敏感性受遗传限制，而静脉注射诱导的耐受性在豚鼠中不受控制。这种耐受性在大多数动物中是完全的，但在弗氏完全佐剂中被二硝基氯苯（DNCB）逆转，并被环磷酰胺预处理所阻止。静脉注射未结合的半抗原诱导的耐受性在弗氏完全佐剂中不会被DNCB逆转，但会被皮内注射DNP-M phi消除。然而，当弗氏完全佐剂中的DNCB与DNP-M phi同时应用时，这种消除不会发生。在进一步的实验中，证明了DNP-M phi诱导接触敏感性的遗传限制在抗原识别水平上，而不是由于用于诱导的巨噬细胞上的Ia结构与参与引发接触敏感性的Ia结构不同。
