二硝基氯苯接触敏感性中的半抗原-细胞缀合物。用二硝基苯基缀合物进行体外刺激可在体内最佳地诱导接触敏感性。
Hapten-cell conjugates in DNCB contact sensitivity. In vitro stimulation with DNP-conjugates optimally inducing contact sensitivity in vivo.
作者信息
von Blomberg M, Scheper R J
出版信息
Immunology. 1980 Feb;39(2):291-9.
Until now in vitro stimulation tests have failed to detect DNCB contact sensitivity in guinea-pigs sensitized epicutaneously without the use of adjuvants. In this study DNP-conjugates optimally inducing contact sensitivity in vivo were tested for their capacity to detect DNCB contact sensitivity in vitro in a lymphocyte transformation assay. In vivo contact sensitivity measurements in guinea-pigs which had been immunized with different hapten-cell conjugates, showed that (1) living cells should be used for conjugation with the allergen, (2) macrophages are optimal carriers and (3) syngeneity is not required. Therefore, DNFB-coated peritoneal macrophages (viable when conjugated) were used as an antigen for in vitro stimulation. Using this conjugate, a highly reproducible antigen-specific increase in DNA synthesis could be obtained in lymph node lymphocytes from guinea-pigs that had been sensitized to DNCB by epicutaneous application of the allergen without the use of adjuvants.
迄今为止，在不使用佐剂的情况下经皮致敏的豚鼠，体外刺激试验未能检测到其对二硝基氯苯（DNCB）的接触敏感性。在本研究中，对在体内能最佳诱导接触敏感性的二硝基苯（DNP）缀合物，在淋巴细胞转化试验中检测其体外检测DNCB接触敏感性的能力。对用不同半抗原-细胞缀合物免疫的豚鼠进行体内接触敏感性测量，结果表明：（1）应使用活细胞与变应原结合；（2）巨噬细胞是最佳载体；（3）不需要基因同质性。因此，用二硝基氟苯（DNFB）包被的腹膜巨噬细胞（结合时 viable）用作体外刺激的抗原。使用这种缀合物，通过经皮施用变应原而不使用佐剂致敏的豚鼠的淋巴结淋巴细胞中，可获得高度可重复的抗原特异性DNA合成增加。
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