一步间接迁移抑制（LIF）试验。

One-step indirect migration inhibiton (LIF) assay.

作者信息

Kalmár L, Gergely P

出版信息

J Immunol Methods. 1982 Sep 17;53(2):245-50. doi: 10.1016/0022-1759(82)90146-6.

Abstract

A simple, 1-step indirect leukocyte migration inhibition (LIF) assay is described. Advantages of this method are as follows: the mitogen does not directly influence the migration of polymorphonuclear leukocytes, relatively few cells are needed, and results are obtained within 24 h. LIF is simultaneously produced by Con A-pretreated lymphocytes in the migration test system. The lymphokine-producing cells are in the test plate well, in the culture medium surrounding the agarose droplets containing migrating cells. The LIF production can be inhibited by the protein synthesis inhibitor puromycin and by prostaglandin (PGE1). In this test system the LIF production is not influenced by endogenous prostaglandins. This method can be used for complex immunopharmacological investigations because effects of agents on the random migration of polymorphonuclear leukocytes and on LIF production of lymphocytes can be tested in the same system.

摘要

本文描述了一种简单的一步间接白细胞迁移抑制（LIF）试验。该方法的优点如下：促有丝分裂原不直接影响多形核白细胞的迁移，所需细胞相对较少，且在24小时内即可获得结果。在迁移试验系统中，伴刀豆球蛋白A预处理的淋巴细胞可同时产生LIF。产生淋巴因子的细胞位于试验板孔中，即在含有迁移细胞的琼脂糖滴周围的培养基中。蛋白质合成抑制剂嘌呤霉素和前列腺素（PGE1）可抑制LIF的产生。在该试验系统中，LIF的产生不受内源性前列腺素的影响。该方法可用于复杂的免疫药理学研究，因为在同一系统中可以测试药物对多形核白细胞随机迁移和淋巴细胞LIF产生的影响。